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dc.contributor.authorFerrari, Giada
dc.contributor.authorCuevas, Angelica
dc.contributor.authorGondek, Agata
dc.contributor.authorBallantyne, Rachel
dc.contributor.authorKersten, Oliver
dc.contributor.authorPalsdottir, Albina Hulda
dc.contributor.authorvan der Jagt, Inge
dc.contributor.authorHufthammer, Anne Karin
dc.contributor.authorYstgaard, Ingrid
dc.contributor.authorWickler, Stephen
dc.contributor.authorBigelow, Gerald F
dc.contributor.authorHarland, Jennifer
dc.contributor.authorNicholson, Rebecca
dc.contributor.authorOrton, David
dc.contributor.authorClavel, Benoît
dc.contributor.authorBoessenkool, Sanne
dc.contributor.authorBarrett, James H
dc.contributor.authorStar, Bastiaan
dc.date.accessioned2021-08-12T07:59:54Z
dc.date.available2021-08-12T07:59:54Z
dc.date.created2021-01-16T00:08:39Z
dc.date.issued2021
dc.identifier.issn0305-4403
dc.identifier.urihttps://hdl.handle.net/11250/2767454
dc.description.abstractThe field of ancient DNA is dominated by studies focusing on terrestrial vertebrates. This taxonomic bias limits our understanding of endogenous DNA preservation for species with different bone physiology, such as teleost fish. Teleost bone is typically brittle, porous, lightweight, and is characterized by a lack of bone remodeling during growth. All of these factors potentially affect DNA preservation. Using high-throughput shotgun sequencing, we here investigate the preservation of DNA in a range of different bone elements from over 200 archaeological Atlantic cod (Gadus morhua) specimens from 38 sites in northern Europe, dating up to 8000 years before present. We observe that the majority of archaeological sites (79%) yield endogenous DNA, with 40% of sites providing samples containing high levels (>20%). Library preparation success and levels of endogenous DNA depend mainly on excavation site and pre-extraction laboratory treatment. The use of pre-extraction treatments lowers the rate of libraries that can be sequenced, although — if successful — the fraction of endogenous DNA can be improved by several orders of magnitude. This trade-off between library preparation success and levels of endogenous DNA allows for alternative extraction strategies depending on the requirements of down-stream analyses and research questions. Finally, we do not find particular bone elements to yield higher levels of endogenous DNA, as is the case for denser bones in mammals. Our results highlight the potential of archaeological fish bone as a source for ancient DNA and suggest a possible role of bone remodeling in the preservation of endogenous DNA.en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleThe preservation of ancient DNA in archaeological fish boneen_US
dc.typeJournal articleen_US
dc.typePeer revieweden_US
dc.description.versionpublishedVersionen_US
dc.rights.holderCopyright 2021 The Authors.en_US
dc.source.articlenumber105317en_US
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode2
dc.identifier.doi10.1016/j.jas.2020.105317
dc.identifier.cristin1872450
dc.source.journalJournal of Archaeological Scienceen_US
dc.relation.projectNorges forskningsråd: 262777en_US
dc.relation.projectNotur/NorStore: NS9244Ken_US
dc.relation.projectNotur/NorStore: NS9003Ken_US
dc.identifier.citationJournal of Archaeological Science. 2021, 126, 105317.en_US
dc.source.volume126en_US


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Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal