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dc.contributor.authorPuppo, Francesca
dc.contributor.authorSadegh, Sanaz
dc.contributor.authorTrujillo, Cleber A.
dc.contributor.authorThunemann, Martin
dc.contributor.authorCampbell, Evan P.
dc.contributor.authorVandenberghe, Matthieu
dc.contributor.authorShan, Xiwei
dc.contributor.authorAkkouh, Ibrahim
dc.contributor.authorMiller, Evan W.
dc.contributor.authorBloodgood, Brenda L.
dc.contributor.authorSilva, Gabriel A.
dc.contributor.authorDale, Anders M.
dc.contributor.authorEinevoll, Gaute
dc.contributor.authorDjurovic, Srdjan
dc.contributor.authorAndreassen, Ole Andreas
dc.contributor.authorMuotri, Alysson R.
dc.contributor.authorDevor, Anna
dc.date.accessioned2021-08-26T06:35:41Z
dc.date.available2021-08-26T06:35:41Z
dc.date.created2021-06-28T12:07:51Z
dc.date.issued2021
dc.identifier.issn1662-5102
dc.identifier.urihttps://hdl.handle.net/11250/2771288
dc.description.abstractVoltage imaging and “all-optical electrophysiology” in human induced pluripotent stem cell (hiPSC)-derived neurons have opened unprecedented opportunities for high-throughput phenotyping of activity in neurons possessing unique genetic backgrounds of individual patients. While prior all-optical electrophysiology studies relied on genetically encoded voltage indicators, here, we demonstrate an alternative protocol using a synthetic voltage sensor and genetically encoded optogenetic actuator that generate robust and reproducible results. We demonstrate the functionality of this method by measuring spontaneous and evoked activity in three independent hiPSC-derived neuronal cell lines with distinct genetic backgrounds.en_US
dc.language.isoengen_US
dc.publisherFrontiers Mediaen_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleAll-Optical Electrophysiology in hiPSC-Derived Neurons With Synthetic Voltage Sensorsen_US
dc.typeJournal articleen_US
dc.typePeer revieweden_US
dc.description.versionpublishedVersionen_US
dc.rights.holderCopyright 2021 Puppo, Sadegh, Trujillo, Thunemann, Campbell, Vandenberghe, Shan, Akkouh, Miller, Bloodgood, Silva, Dale, Einevoll, Djurovic, Andreassen, Muotri and Devoren_US
dc.source.articlenumber671549en_US
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1
dc.identifier.doi10.3389/fncel.2021.671549
dc.identifier.cristin1918860
dc.source.journalFrontiers in Cellular Neuroscienceen_US
dc.identifier.citationFrontiers in Cellular Neuroscience. 2021, 15, 671549.en_US
dc.source.volume15en_US


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Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal