dc.contributor.author | Edwards, David | |
dc.contributor.author | Røyrvik, Ellen Christine | |
dc.contributor.author | Chustecki, Joanne | |
dc.contributor.author | Giannakis, Konstantinos | |
dc.contributor.author | Glastad, Robert Clay | |
dc.contributor.author | Radzvilavicius, Arunas | |
dc.contributor.author | Johnston, Iain | |
dc.date.accessioned | 2022-03-07T09:38:20Z | |
dc.date.available | 2022-03-07T09:38:20Z | |
dc.date.created | 2022-01-15T10:17:31Z | |
dc.date.issued | 2021 | |
dc.identifier.issn | 1544-9173 | |
dc.identifier.uri | https://hdl.handle.net/11250/2983335 | |
dc.description.abstract | Mitochondrial DNA (mtDNA) and plastid DNA (ptDNA) encode vital bioenergetic apparatus, and mutations in these organelle DNA (oDNA) molecules can be devastating. In the germline of several animals, a genetic “bottleneck” increases cell-to-cell variance in mtDNA heteroplasmy, allowing purifying selection to act to maintain low proportions of mutant mtDNA. However, most eukaryotes do not sequester a germline early in development, and even the animal bottleneck remains poorly understood. How then do eukaryotic organelles avoid Muller’s ratchet—the gradual buildup of deleterious oDNA mutations? Here, we construct a comprehensive and predictive genetic model, quantitatively describing how different mechanisms segregate and decrease oDNA damage across eukaryotes. We apply this comprehensive theory to characterise the animal bottleneck with recent single-cell observations in diverse mouse models. Further, we show that gene conversion is a particularly powerful mechanism to increase beneficial cell-to-cell variance without depleting oDNA copy number, explaining the benefit of observed oDNA recombination in diverse organisms which do not sequester animal-like germlines (for example, sponges, corals, fungi, and plants). Genomic, transcriptomic, and structural datasets across eukaryotes support this mechanism for generating beneficial variance without a germline bottleneck. This framework explains puzzling oDNA differences across taxa, suggesting how Muller’s ratchet is avoided in different eukaryotes. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Public Library of Science | en_US |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.title | Avoiding organelle mutational meltdown across eukaryotes with or without a germline bottleneck | en_US |
dc.type | Journal article | en_US |
dc.type | Peer reviewed | en_US |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | Copyright 2021 Edwards et al. | en_US |
dc.source.articlenumber | e3001153 | en_US |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 2 | |
dc.identifier.doi | 10.1371/journal.pbio.3001153 | |
dc.identifier.cristin | 1981709 | |
dc.source.journal | PLoS Biology | en_US |
dc.relation.project | ERC-European Research Council: 805046 | en_US |
dc.identifier.citation | PLoS Biology. 2021, 19 (4), e3001153. | en_US |
dc.source.volume | 19 | en_US |
dc.source.issue | 4 | en_US |