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dc.contributor.authorGutiérrez-García, Claudia
dc.contributor.authorAhmed, Shiek S. S. J.
dc.contributor.authorRamalingam, Sathishkumar
dc.contributor.authorSelvaraj, Dhivya
dc.contributor.authorSrivastava, Aashish
dc.contributor.authorPaul, Sujay
dc.contributor.authorSharma, Ashutosh
dc.date.accessioned2022-08-12T11:23:39Z
dc.date.available2022-08-12T11:23:39Z
dc.date.created2022-05-09T12:41:01Z
dc.date.issued2022
dc.identifier.issn2223-7747
dc.identifier.urihttps://hdl.handle.net/11250/3011619
dc.description.abstractMicroRNAs (miRNAs) are small noncoding RNA molecules that play crucial post-transcriptional regulatory roles in plants, including development and stress-response signaling. However, information about their involvement in secondary metabolism is still limited. Murraya koenigii is a popular medicinal plant, better known as curry leaves, that possesses pharmaceutically active secondary metabolites. The present study utilized high-throughput sequencing technology to investigate the miRNA profile of M. koenigii and their association with secondary metabolite biosynthesis. A total of 343,505 unique reads with lengths ranging from 16 to 40 nt were obtained from the sequencing data, among which 142 miRNAs were identified as conserved and 7 as novel miRNAs. Moreover, 6078 corresponding potential target genes of M. koenigii miRNAs were recognized in this study. Interestingly, several conserved and novel miRNAs of M. koenigii were found to target key enzymes of the terpenoid backbone and the flavonoid biosynthesis pathways. Furthermore, to validate the sequencing results, the relative expression of eight randomly selected miRNAs was determined by qPCR. To the best of our knowledge, this is the first report of the M. koenigii miRNA profile that may provide useful information for further elucidation of the involvement of miRNAs in secondary metabolism. These findings might be crucial in the future to generate artificial-miRNA-based, genetically engineered M. koenigii plants for the overproduction of medicinally highly valuable secondary metabolites.en_US
dc.language.isoengen_US
dc.publisherMDPIen_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleIdentification of microRNAs from medicinal plant Murraya Koenigii by high-throughput sequencing and their functional implications in secondary metabolite biosynthesisen_US
dc.typeJournal articleen_US
dc.typePeer revieweden_US
dc.description.versionpublishedVersionen_US
dc.rights.holderCopyright 2022 The Author(s)en_US
dc.source.articlenumber46en_US
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1
dc.identifier.doi10.3390/plants11010046
dc.identifier.cristin2022651
dc.source.journalPlantsen_US
dc.identifier.citationPlants. 2022, 11 (1), 46.en_US
dc.source.volume11en_US
dc.source.issue1en_US


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