dc.contributor.author | Keindl, Magdalena | |
dc.contributor.author | Davies, Richard Allan | |
dc.contributor.author | Bergum, Brith | |
dc.contributor.author | Brun, Johan Gorgas | |
dc.contributor.author | Hammenfors, Daniel | |
dc.contributor.author | Jonsson, Roland | |
dc.contributor.author | Lyssenko, Valeriya | |
dc.contributor.author | Appel, Silke | |
dc.date.accessioned | 2022-10-25T10:57:35Z | |
dc.date.available | 2022-10-25T10:57:35Z | |
dc.date.created | 2022-10-13T20:54:42Z | |
dc.date.issued | 2022 | |
dc.identifier.issn | 1478-6354 | |
dc.identifier.uri | https://hdl.handle.net/11250/3028168 | |
dc.description.abstract | Background
Interleukin-2 (IL-2) and the high-affinity IL-2 receptor (IL-2R) are essential for the survival of regulatory T cells (Tregs) which are the main players in immune tolerance and prevention of autoimmune diseases. Sjögren’s syndrome (SS) is a chronic autoimmune disease predominantly affecting women and is characterised by sicca symptoms including oral and ocular dryness. The aim of this study was to investigate an association between IL-2R and Treg function in patients with SS of different severity defined by the salivary flow rate.
Methods
In a cross-sectional study, we determined plasma soluble IL-2R (sIL-2R) levels in women with SS (n=97) and healthy females (n=50) using ELISA. A subset of those (n=51) was screened for Treg function measured by the STAT5 signalling response to IL-2 using phospho-flow cytometry.
Results
We found that elevated plasma levels of sIL-2R were positively associated with the severity of SS reflected by a pathologically low salivary flow. Phospho-flow analysis revealed that patients with SS have a significantly lower frequency of pSTAT5+ Tregs upon IL-2 stimulation compared with healthy individuals, while the frequency of Tregs and pSTAT5 in conventional T cells remained unchanged. In addition, we observed more pSTAT5+ Tregs at baseline in patients with SS, which is significantly associated with seropositivity and elevated sIL-2R.
Conclusions
Our data indicates that Tregs have a weakened immunosuppressive function in patients with SS due to impaired IL-2/IL-2R signalling capacity. This could mediate lymphocytic infiltration into salivary glands inducing sicca symptoms. We believe that sIL-2R could act as a useful indicator for SS and disease severity. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | BMC | en_US |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.title | Impaired activation of STAT5 upon IL-2 stimulation in Tregs and elevated sIL-2R in Sjögren’s syndrome | en_US |
dc.type | Journal article | en_US |
dc.type | Peer reviewed | en_US |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | Copyright 2022 The Author(s) | en_US |
dc.source.articlenumber | 101 | en_US |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 2 | |
dc.identifier.doi | 10.1186/s13075-022-02769-y | |
dc.identifier.cristin | 2061327 | |
dc.source.journal | Arthritis Research & Therapy | en_US |
dc.identifier.citation | Arthritis Research & Therapy. 2022, 24, 101. | en_US |
dc.source.volume | 24 | en_US |