dc.contributor.author | Tran, Trung The | |
dc.contributor.author | Vaage, Eline Benno | |
dc.contributor.author | Mehta, Adi | |
dc.contributor.author | Chopra, Adity | |
dc.contributor.author | Tietze, Lisa | |
dc.contributor.author | Kolderup, Anette | |
dc.contributor.author | Anthi, Aina Karen | |
dc.contributor.author | König, Marton | |
dc.contributor.author | Nygaard, Gro Owren | |
dc.contributor.author | Lind, Andreas | |
dc.contributor.author | Müller, Fredrik | |
dc.contributor.author | Nissen-Meyer, Lise Sofie Haug | |
dc.contributor.author | Magnus, Per Minor | |
dc.contributor.author | Trogstad, Lill | |
dc.contributor.author | Mjaaland, Siri | |
dc.contributor.author | Søraas, Arne Vasli | |
dc.contributor.author | Midtvedt, Karsten | |
dc.contributor.author | Åsberg, Anders | |
dc.contributor.author | Barratt-Due, Andreas | |
dc.contributor.author | Medhus, Asle Wilhelm | |
dc.contributor.author | Høivik, Marte Lie | |
dc.contributor.author | Lundin, Knut | |
dc.contributor.author | Karlsen, Randi Fuglaas | |
dc.contributor.author | Dahle, Reidun | |
dc.contributor.author | Danielsson, Karin | |
dc.contributor.author | Thomassen, Kristine Stien | |
dc.contributor.author | Kro, Grete Anette Birkeland | |
dc.contributor.author | Cox, Rebecca Jane | |
dc.contributor.author | Zhou, Fan | |
dc.contributor.author | Langeland, Nina | |
dc.contributor.author | Aukrust, Pål | |
dc.contributor.author | Melum, Espen | |
dc.contributor.author | Åvitsland, Tone Lise | |
dc.contributor.author | Wiencke, Kristine | |
dc.contributor.author | Holter, Jan Cato | |
dc.contributor.author | Munthe, Ludvig Andre | |
dc.contributor.author | Grødeland, Gunnveig | |
dc.contributor.author | Andersen, Jan Terje | |
dc.contributor.author | Vaage, John T. | |
dc.contributor.author | Lund-Johansen, Fridtjof | |
dc.date.accessioned | 2023-01-13T09:32:11Z | |
dc.date.available | 2023-01-13T09:32:11Z | |
dc.date.created | 2023-01-04T16:05:38Z | |
dc.date.issued | 2022 | |
dc.identifier.issn | 2059-0105 | |
dc.identifier.uri | https://hdl.handle.net/11250/3043266 | |
dc.description.abstract | Diagnostic assays currently used to monitor the efficacy of COVID-19 vaccines measure levels of antibodies to the receptor-binding domain of ancestral SARS-CoV-2 (RBDwt). However, the predictive value for protection against new variants of concern (VOCs) has not been firmly established. Here, we used bead-based arrays and flow cytometry to measure binding of antibodies to spike proteins and receptor-binding domains (RBDs) from VOCs in 12,000 serum samples. Effects of sera on RBD-ACE2 interactions were measured as a proxy for neutralizing antibodies. The samples were obtained from healthy individuals or patients on immunosuppressive therapy who had received two to four doses of COVID-19 vaccines and from COVID-19 convalescents. The results show that anti-RBDwt titers correlate with the levels of binding- and neutralizing antibodies against the Alpha, Beta, Gamma, Delta, Epsilon and Omicron variants. The benefit of multiplexed analysis lies in the ability to measure a wide range of anti-RBD titers using a single dilution of serum for each assay. The reactivity patterns also yield an internal reference for neutralizing activity and binding antibody units per milliliter (BAU/ml). Results obtained with sera from vaccinated healthy individuals and patients confirmed and extended results from previous studies on time-dependent waning of antibody levels and effects of immunosuppressive agents. We conclude that anti-RBDwt titers correlate with levels of neutralizing antibodies against VOCs and propose that our method may be implemented to enhance the precision and throughput of immunomonitoring. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Nature Research | en_US |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.title | Titers of antibodies against ancestral SARS-CoV-2 correlate with levels of neutralizing antibodies to multiple variants | en_US |
dc.type | Journal article | en_US |
dc.type | Peer reviewed | en_US |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | Copyright The Author(s) 2022 | en_US |
dc.source.articlenumber | 174 | en_US |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.doi | 10.1038/s41541-022-00586-7 | |
dc.identifier.cristin | 2100792 | |
dc.source.journal | npj Vaccines | en_US |
dc.identifier.citation | npj Vaccines. 2022, 7, 174. | en_US |
dc.source.volume | 7 | en_US |