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dc.contributor.authorRim, Sehee
dc.contributor.authorSakkestad, Sunniva Todnem
dc.contributor.authorZhou, Fan
dc.contributor.authorGullaksen, Stein-Erik
dc.contributor.authorSkavland, Jørn
dc.contributor.authorChauhan, Sudhir Kumar
dc.contributor.authorSteinsland, Hans
dc.contributor.authorHanevik, Kurt
dc.description.abstractEnterotoxigenic Escherichia coli (ETEC) is an important cause of children's and travelers’ diarrhea, with no licensed vaccine. This study aimed to explore the role of cellular immunity in protection against human ETEC infection. Nine volunteers were experimentally infected with ETEC, of which six developed diarrhea. Lymphocytes were collected from peripheral blood buffy coats, before and 3, 5, 6, 7, 10, and 28 days after dose ingestion, and 34 phenotypic and functional markers were examined by mass cytometry. Thirty-three cell populations, derived by manually merging 139 cell clusters from the X-shift unsupervised clustering algorithm, were analyzed. Initially, the diarrhea group responded with increased CD56dim CD16+ natural killer cells, dendritic cells tended to rise, and mucosal-associated invariant T cells decreased. On day 5-7, an increase in plasmablasts was paralleled by a consistent rise in CD4+ Th17-like effector memory and regulatory cell subsets. CD4+ Th17-like central memory cells peaked on day 10. All Th17-like cell populations showed increased expression of activation, gut-homing, and proliferation markers. Interestingly, in the nondiarrhea group, these same CD4+ Th17-like cell populations expanded earlier, normalizing around day 7. Earlier development of these CD4+ Th17-like cell populations in the nondiarrhea group may suggest a recall response and a potential role in controlling ETEC infections.en_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.titleDynamics of circulating lymphocytes responding to human experimental enterotoxigenic Escherichia coli infectionen_US
dc.typeJournal articleen_US
dc.typePeer revieweden_US
dc.rights.holderCopyright 2023 The Author(s)en_US
dc.source.journalEuropean Journal of Immunologyen_US
dc.identifier.citationEuropean Journal of Immunology. 2023, 53 (8), 2250254.en_US

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Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal