Exploring the impact of thermal delousing on gill health and microbiome dynamics in farmed Atlantic Salmon

Abstract

The economic cost of salmon louse (Lepeophtheirus salmonis and Caligus rogercresseyi) infestations in the Atlantic salmon (Salmo salar) industry has been estimated to be around $900 million annually. This high cost has driven a concerted effort to develop, examine, understand, and implement various methods for louse control. Husbandry interventions utilising warm water exposure have been highly successful in complementing traditional chemotherapeutants, especially as the efficiency of the latter has reduced in recent years. In this study, we sought to examine the impact of thermal delousing on gill health in two commercial sites with different historical husbandry and treatment interventions prior to and post-thermal treatment. Methods to characterise gill health and the response to thermal treatment included a detailed examination of the gill for microparasites using both histology and qPCR and targeted immune gene expression analysis, most notably antigen-presenting cells (mhc ii), proinflammatory cytokines (il-1β and tnf-α) and inhibitory cytokines (tgf-β and il-10). Furthermore, we examined the bacterial communities present on the gill surface using 16S rRNA amplicon sequencing. Data obtained from these trials indicated a minimal impact on gill microparasite prevalence in response to the thermal treatment. The expression of immune markers exhibited a significant decrease across both sites after treatment. Intriguingly, marked differences in the gill bacterial communities in response to treatment between the two sites were clearly observed. This divergence could be attributed to the notable differences in husbandry history and health status of the fish at the two sites prior to the thermal treatment. Our data suggest that microbiome diversity is an informative indicator of fish gill health and could be used to define appropriate interventions when treating sea lice.