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dc.contributor.authorAarstad, Helene Hersviken_US
dc.contributor.authorVintermyr, Olav Karstenen_US
dc.contributor.authorUlvestad, Ellingen_US
dc.contributor.authorKross, Kenneth Wilfrieden_US
dc.contributor.authorHeimdal, John-Helgeen_US
dc.contributor.authorAarstad, Hans Jørgenen_US
dc.date.accessioned2016-06-01T08:37:09Z
dc.date.available2016-06-01T08:37:09Z
dc.date.issued2015-06-16
dc.PublishedPLoS ONE 2015, 10:e0129724(6)eng
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/1956/12044
dc.description.abstractThe study was performed in order to determine whether peripheral blood monocyte in vitro function, and lymphocyte in vivo activation at diagnosis, was associated with HPV tumor infection status and 15-year survival in head and neck squamous cell carcinoma (HNSCC) patients. Sixty-five patients from a consecutive cohort of newly diagnosed HNSCCs, together with 18 control patients, were included in the study. Monocyte responsiveness was assessed by measuring monocyte in vitro interleukin (IL)-6 secretions after 24 hours of LPS stimulation in cultures with a serum-free medium. T lymphocyte activation was determined as the fraction of CD71-positive cells on CD3-positive cells by flow cytometry, whereas HPV infection was determined by PCR on formalin-fixed paraffin-embedded (FFPE) tumor tissue. Disease-specific survivals and overall survivals were determined 15 years following inclusion. HPV-positive HNSCC patients had a lower monocyte LPS-stimulated IL-6 response. A high LPS-stimulated monocyte IL-6 response predicted a decreased survival rate (P=0.019). A high percentage of CD71-positive T lymphocytes also predicted an impaired prognosis (P=0.021). The predictive power of IL-6 monocyte LPS-stimulated responses was retained when adjusted for age, gender and TNM stage of the patients. The monocyte and T lymphocyte survival predictions were independent of each other. The survival was particularly low with a combined high activated monocyte and T lymphocyte status. In a multivariate analysis, IL-6 secretion and the percentage of CD71-positive T lymphocytes both uniquely predicted survival independent of HPV infection status. It is postulated that the natural and adaptive immune systems are separately and additionally linked to the clinical aggressiveness of HNSCCs.en_US
dc.language.isoengeng
dc.publisherPLOS ONEeng
dc.rightsAttribution CC BY 4.0eng
dc.rights.urihttp://creativecommons.org/licenses/by/4.0eng
dc.titleIn vitro-stimulated IL-6 monocyte secretion and in vivo peripheral blood T lymphocyte activation uniquely predicted 15-year survival in patients with head and neck squamous cell carcinomaen_US
dc.typePeer reviewed
dc.typeJournal article
dc.date.updated2016-03-31T13:45:28Z
dc.description.versionpublishedVersionen_US
dc.rights.holderCopyright 2015 Aarstad et al.
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0129724
dc.identifier.cristin1275959
dc.subject.nsiVDP::Medisinske fag: 700::Helsefag: 800::Epidemiologi medisinsk og odontologisk statistikk: 803
dc.subject.nsiVDP::Midical sciences: 700::Health sciences: 800::Epidemiology, medical and dental statistics: 803
dc.subject.nsiVDP::Medisinske Fag: 700en_US


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Except where otherwise noted, this item's license is described as Attribution CC BY 4.0