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dc.contributor.authorMohn, Kristin Greve-Isdahlen_US
dc.contributor.authorBrokstad, Karl Alberten_US
dc.contributor.authorPathirana, Rishien_US
dc.contributor.authorBredholt, Geiren_US
dc.contributor.authorJul-Larsen, Åsneen_US
dc.contributor.authorTrieu, Mai Chien_US
dc.contributor.authorJalloh, Sarah Larteley Larteyen_US
dc.contributor.authorMontemoli, Emanueleen_US
dc.contributor.authorTøndel, Camillaen_US
dc.contributor.authorAarstad, Hans Jørgenen_US
dc.contributor.authorCox, Rebecca Janeen_US
dc.PublishedJournal of Infectious Diseases 2016, 214(5):722-731eng
dc.description.abstractBackground. Tonsils play a key role in eliciting immune responses against respiratory pathogens. Little is known about how tonsils contribute to the local immune response after intranasal vaccination. Here, we uniquely report the mucosal humoral responses in tonsils and saliva after intranasal live attenuated influenza vaccine (LAIV) vaccination in children. Methods. Blood, saliva, and tonsils samples were collected from 39 children before and after LAIV vaccination and from 16 age-matched, nonvaccinated controls. Serum antibody responses were determined by a hemagglutination inhibition (HI) assay. The salivary immunoglobulin A (IgA) level was measured by an enzyme-linked immunosorbent assay. Antibody-secreting cell (ASC) and memory B-cell (MBC) responses were enumerated in tonsils and blood. Results. Significant increases were observed in levels of serum antibodies and salivary IgA to influenza A(H3N2) and influenza B virus strains as early as 14 days after vaccination but not to influenza A(H1N1). Influenza virus–specific salivary IgA levels correlated with serum HI responses, making this a new possible indicator of vaccine immunogenicity in children. LAIV augmented influenza virus–specific B-cell responses in tonsils and blood. Tonsillar MBC responses correlated with systemic MBC and serological responses. Naive children showed significant increases in MBC counts after LAIV vaccination. Conclusions. This is the first study to demonstrate that LAIV elicits humoral B-cell responses in tonsils of young children. Furthermore, salivary IgA analysis represents an easy method for measuring immunogenicity after vaccination.en_US
dc.publisherOxford University Presseng
dc.rightsAttribution CC BY-NC-NDeng
dc.subjectlymphoid tissueeng
dc.subjectsaliva immune responseeng
dc.subjectmemory B celleng
dc.subjectantibody-secreting celleng
dc.titleLive attenuated influenza vaccine in children induces b-cell responses in tonsilsen_US
dc.typePeer reviewed
dc.typeJournal article
dc.rights.holderCopyright 2016 The Author(s)
dc.identifier.citationJournal of Infectious Diseases 2016, 214 (5), 722-731.

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