dc.contributor.author | Konstantinova, Victoria | en_US |
dc.contributor.author | Ibrahim, Mohamed | en_US |
dc.contributor.author | Lie, Stein Atle | en_US |
dc.contributor.author | Birkeland Salmorin, Eivind | en_US |
dc.contributor.author | Neppelberg, Evelyn | en_US |
dc.contributor.author | Marthinussen Cuida, Mihaela | en_US |
dc.contributor.author | Costea, Daniela Elena | en_US |
dc.contributor.author | Cimpan, Mihaela Roxana | en_US |
dc.date.accessioned | 2017-01-02T13:27:18Z | |
dc.date.available | 2017-01-02T13:27:18Z | |
dc.date.issued | 2016 | |
dc.Published | Journal of Oral Pathology & Medicine 2016 | eng |
dc.identifier.issn | 1600-0714 | |
dc.identifier.uri | https://hdl.handle.net/1956/15331 | |
dc.description.abstract | BACKGROUND: Oral cavity is a doorway for a variety of products containing titanium dioxide (TiO2) nanoparticles (NPs) (nano-TiO2) such as food additives, oral healthcare products and dental materials. Their potential to penetrate and affect normal human oral mucosa is not yet determined. OBJECTIVES: To evaluate the ability of nano-TiO2 to penetrate the in vitro reconstructed normal human buccal mucosa (RNHBM). METHODS: RNHBM was generated from primary normal human oral keratinocytes and fibroblasts isolated from buccal oral mucosa of healthy patients (n = 6). The reconstructed tissues were exposed after 10 days to clinically relevant concentrations of spherical or spindle rutile nano-TiO2 in suspension for short (20 min) and longer time (24 h). Ultrahigh-resolution imaging (URI) microscopy (CytoVivaTM, Auburn, AL, USA) was used to assess the depth of penetration into reconstructed tissues. RESULTS: Ultrahigh-resolution imaging microscopy demonstrated the presence of nano-TiO2 mostly in the epithelium of RNHBM at both 20 min and 24-h exposure, and this was shape and doze dependent at 24 h of exposure. The depth of penetration diminished in time at higher concentrations. The exposed epithelium showed increased desquamation but preserved thickness. CONCLUSION: Nano-TiO2 is able to penetrate RNHBM and to activate its barrier function in a doze- and timedependent manner. | en_US |
dc.language.iso | eng | eng |
dc.publisher | Wiley | eng |
dc.rights | Attribution CC BY | eng |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | eng |
dc.subject | epithelium | eng |
dc.subject | nanoparticles | eng |
dc.subject | oral | eng |
dc.subject | organotypic model | eng |
dc.subject | titanium dioxide | eng |
dc.title | Nano-TiO2 penetration of oral mucosa: in vitro analysis using 3D organotypic human buccal mucosa models | en_US |
dc.type | Peer reviewed | |
dc.type | Journal article | |
dc.date.updated | 2016-12-15T10:46:22Z | |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | Copyright 2016 The Author(s) | |
dc.identifier.doi | https://doi.org/10.1111/jop.12469 | |
dc.identifier.cristin | 1375868 | |