Vis enkel innførsel

dc.contributor.authorAndersen, Tor Kristianen_US
dc.contributor.authorZhou, Fanen_US
dc.contributor.authorCox, Rebecca Janeen_US
dc.contributor.authorBogen, Bjarneen_US
dc.contributor.authorGrødeland, Gunnveigen_US
dc.date.accessioned2018-04-11T12:06:41Z
dc.date.available2018-04-11T12:06:41Z
dc.date.issued2017-12
dc.PublishedAndersen TK, Zhou F, Cox RJ, Bogen B, Grødeland G. A DNA vaccine that targets hemagglutinin to antigen-presenting cells protects mice against H7 influenza. Journal of Virology. 2017;91:e01340-17eng
dc.identifier.issn0022-538X
dc.identifier.issn1098-5514
dc.identifier.urihttps://hdl.handle.net/1956/17603
dc.description.abstractZoonotic influenza H7 viral infections have a case fatality rate of about 40%. Currently, no or limited human to human spread has occurred, but we may be facing a severe pandemic threat if the virus acquires the ability to transmit between humans. Novel vaccines that can be rapidly produced for global distribution are urgently needed, and DNA vaccines may be the only type of vaccine that allows for the speed necessary to quench an emerging pandemic. Here, we constructed DNA vaccines encoding the hemagglutinin (HA) from influenza A/chicken/Italy/13474/99 (H7N1). In order to increase the efficacy of DNA vaccination, HA was targeted to either major histocompatibility complex class II molecules or chemokine receptors 1, 3, and 5 (CCR1/3/5) that are expressed on antigen-presenting cells (APC). A single DNA vaccination with APC-targeted HA significantly increased antibody levels in sera compared to nontargeted control vaccines. The antibodies were confirmed neutralizing in an H7 pseudotype-based neutralization assay. Furthermore, the APC-targeted vaccines increased the levels of antigen-specific cytotoxic T cells, and a single DNA vaccination could confer protection against a lethal challenge with influenza A/turkey/Italy/3889/1999 (H7N1) in mice. In conclusion, we have developed a vaccine that rapidly could contribute protection against a pandemic threat from avian influenza. IMPORTANCE Highly pathogenic avian influenza H7 constitute a pandemic threat that can cause severe illness and death in infected individuals. Vaccination is the main method of prophylaxis against influenza, but current vaccine strategies fall short in a pandemic situation due to a prolonged production time and insufficient production capabilities. In contrast, a DNA vaccine can be rapidly produced and deployed to prevent the potential escalation of a highly pathogenic influenza pandemic. We here demonstrate that a single DNA delivery of hemagglutinin from an H7 influenza could mediate full protection against a lethal challenge with H7N1 influenza in mice. Vaccine efficacy was contingent on targeting of the secreted vaccine protein to antigen-presenting cells.en_US
dc.language.isoengeng
dc.publisherAmerican Society for Microbiologyeng
dc.rightsAttribution CC BYeng
dc.rights.urihttp://creativecommons.org/licenses/by/4.0eng
dc.subjectAPC-targetingeng
dc.subjectDNA vaccineeng
dc.subjectavian viruseseng
dc.subjecthemagglutinineng
dc.subjectinfluenzaeng
dc.subjectpandemic influenzaeng
dc.titleA DNA vaccine that targets hemagglutinin to antigen-presenting cells protects mice against H7 influenzaen_US
dc.typePeer reviewed
dc.typeJournal article
dc.date.updated2018-01-24T14:36:46Z
dc.description.versionpublishedVersionen_US
dc.rights.holderCopyright 2017 The Author(s)
dc.identifier.doihttps://doi.org/10.1128/jvi.01340-17
dc.identifier.cristin1527721
dc.source.journalJournal of Virology
dc.relation.projectStiftelsen Kristian Gerhard Jebsen: SKGJ-MED-011


Tilhørende fil(er)

Thumbnail

Denne innførselen finnes i følgende samling(er)

Vis enkel innførsel

Attribution CC BY
Med mindre annet er angitt, så er denne innførselen lisensiert som Attribution CC BY