dc.contributor.author | Steinrücken, Pia | |
dc.contributor.author | Mjøs, Svein Are | |
dc.contributor.author | Prestegard, Siv Kristin | |
dc.contributor.author | Erga, Svein Rune | |
dc.date.accessioned | 2018-05-28T06:58:36Z | |
dc.date.available | 2018-05-28T06:58:36Z | |
dc.date.issued | 2018-04-17 | |
dc.Published | Steinrücken P, Mjøs SA, Prestegard SK, Erga SR. Enhancing EPA Content in an Arctic Diatom: A Factorial Design Study to Evaluate Interactive Effects of Growth Factors. Frontiers in Plant Science. 2018;9:491 | eng |
dc.identifier.issn | 1664-462X | en_US |
dc.identifier.uri | https://hdl.handle.net/1956/17720 | |
dc.description.abstract | Microalgae with a high content of the omega-3 polyunsaturated fatty acids (PUFAs), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) are of great demand for microalgae-based technologies. An Arctic strain of the diatom Attheya septentrionalis was shown in previous experiments to increase its EPA content from 3.0 to 4.6% of dry weight (DW) in the nutrient-replete exponential phase and nutrient-depleted stationary phase, respectively. In the present study, a factorial-design experiment was used, to investigate this effect in more detail and in combination with varying salinities and irradiances. A mathematical model revealed that both growth phase and salinity, alone and in combination, influenced the EPA content significantly. Maximum EPA values of 7.1% DW were obtained at a salinity of 22 and after 5 days in stationary phase, and might be related to a decreased silica content, an accumulation of storage lipids containing EPA, or both. However, growth rates were lower for low salinity (0.54 and 0.57 d−1) than high salinity (0.77 and 0.98 d−1) cultures. | en_US |
dc.language.iso | eng | eng |
dc.publisher | Frontiers | en_US |
dc.relation.ispartof | <a href="http://hdl.handle.net/1956/17722" target="_blank">High-value fatty acids from microalgae. Bioprospecting and outdoor cultivation at northern latitudes</a> | en_US |
dc.rights | Attribution CC BY | eng |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0 | eng |
dc.subject | Eicosapentaenoic acid (EPA) | eng |
dc.subject | arctic diatom | eng |
dc.subject | factorial design | eng |
dc.subject | Salinity | eng |
dc.subject | growth phase | eng |
dc.subject | interactive effects | eng |
dc.subject | microalgal biotechnology | eng |
dc.title | Enhancing EPA Content in an Arctic Diatom: A Factorial Design Study to Evaluate Interactive Effects of Growth Factors | en_US |
dc.type | Peer reviewed | |
dc.type | Journal article | |
dc.date.updated | 2018-05-28T06:54:29Z | |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | Copyright 2018 The Author(s) | en_US |
dc.identifier.doi | https://doi.org/10.3389/fpls.2018.00491 | |
dc.identifier.cristin | 1579768 | |