dc.contributor.author | Olsen, Ranveig Ottøy | |
dc.contributor.author | Hess-Erga, Ole-Kristian | |
dc.contributor.author | Larsen, Aud | |
dc.contributor.author | Hoffmann, Friederike | |
dc.contributor.author | Thuestad, Gunnar | |
dc.contributor.author | Hoell, Ingunn Alne | |
dc.date.accessioned | 2018-07-31T11:02:20Z | |
dc.date.available | 2018-07-31T11:02:20Z | |
dc.date.issued | 2016 | |
dc.Published | Olsen RO, Hess-Erga OH, Larsen A, Hoffmann F, Thuestad G, Hoell I. Dual staining with CFDA-AM and SYTOX Blue in flow cytometry analysis of UV-irradiated Tetraselmis suecica to evaluate vitality. Aquatic Biology. 2016;25:39-52 | eng |
dc.identifier.issn | 1864-7790 | en_US |
dc.identifier.uri | https://hdl.handle.net/1956/17915 | |
dc.description.abstract | After disinfection of ballast water, it is crucial to detect organisms and determine their vitality to assess the performance of the chosen treatment technique. Ultraviolet (UV) irradiation is a treatment technology commonly used for water disinfection. In this study, the phytoplankter Tetraselmis suecica was UV irradiated and subsequently stained with both 5-carboxyfluorescein diacetate acetoxymethyl ester (CFDA-AM) and SYTOX Blue, staining metabolically active and membrane-permeable cells, respectively. This dual staining protocol can be used to assess samples during type approval of UV-based treatment systems. Non-irradiated and UV-irradiated samples were incubated in darkness, to simulate a ballast water transport, after which the vitality and viability T. suecica were monitored regularly over a period of 15 d. Flow cytometry (FCM) analysis separated the cells into 4 FCM populations (=single cells grouped together based on their fluorescence signals) according to differences in esterase activity and membrane integrity. UV-irradiated samples followed a different staining pattern compared to non-irradiated samples, where 1 specific FCM population of cells expressed esterase activity, but at the same time gave signals for disrupted membranes. This is useful as a sign of future death and is interpreted as an ‘early warning’ FCM population. FCM results were also compared to corresponding plate count results, differentiating vital, viable cells from vital, non-viable cells. We argue that dual staining with SYTOX Blue and CFDA-AM facilitates and improves FCM analysis when evaluating the performance of UV-based water treatment systems. | en_US |
dc.language.iso | eng | eng |
dc.publisher | Inter-Research | en_US |
dc.rights | Attribution CC BY | eng |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0 | eng |
dc.subject | Phytoplankton | eng |
dc.subject | Ballast water | eng |
dc.subject | Water treatment | eng |
dc.subject | Live/dead analysis | eng |
dc.subject | Viability | eng |
dc.subject | Water analysis | eng |
dc.title | Dual staining with CFDA-AM and SYTOX Blue in flow cytometry analysis of UV-irradiated Tetraselmis suecica to evaluate vitality | en_US |
dc.type | Peer reviewed | |
dc.type | Journal article | |
dc.date.updated | 2018-04-10T08:39:15Z | |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | Copyright 2016 The Author(s) | en_US |
dc.identifier.doi | https://doi.org/10.3354/ab00662 | |
dc.identifier.cristin | 1392933 | |
dc.source.journal | Aquatic Biology | |
dc.relation.project | Norges forskningsråd: 208653/O70 | |