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dc.contributor.authorTangedal, Solveigen_US
dc.contributor.authorAanerud, Marianneen_US
dc.contributor.authorGrønseth, Runeen_US
dc.contributor.authorDrengenes, Christineen_US
dc.contributor.authorWiker, Harald Gen_US
dc.contributor.authorBakke, Per S.en_US
dc.contributor.authorEagan, Tomas Mikalen_US
dc.PublishedTangedal S, Aanerud M, Grønseth R, Drengenes C, Wiker HG, Bakke PS, Eagan TML. Comparing microbiota profiles in induced and spontaneous sputum samples in COPD patients. Respiratory Research. 2017;18:164eng
dc.description.abstractBackground: Induced and spontaneous sputum are used to evaluate the airways microbiota. Whether the sputum types can be used interchangeably in microbiota research is unknown. Our aim was to compare microbiota in induced and spontaneous sputum from COPD patients sampled during the same consultation. Methods: COPD patients from Bergen, Norway, were followed between 2006/2010, examined during the stable state and exacerbations. 30 patients delivered 36 sample pairs. DNA was extracted by enzymatic and mechanical lysis methods. The V3-V4 region of the 16S rRNA gene was PCR-amplified and prepared for paired-end sequencing. Illumina Miseq System was used for sequencing, and Quantitative Insights Into Microbial Ecology (QIIME) and Stata were used for bioinformatics and statistical analyses. Results: Approximately 4 million sequences were sorted into 1004 different OTUs and further assigned to 106 different taxa. Pair-wise comparison of both taxonomic composition and beta-diversity revealed significant differences in one or both parameters in 1/3 of sample pairs. Alpha-diversity did not differ. Comparing abundances for each taxa identified, showed statistically significant differences between the mean abundances in induced versus spontaneous samples for 15 taxa when disease state was considered. This included potential pathogens like Haemophilus and Moraxella. Conclusion: When studying microbiota in sputum samples one should take into consideration how samples are collected and avoid the usage of both induced and spontaneous sputum in the same studyen_US
dc.publisherBioMed Centraleng
dc.rightsAttribution CC BYeng
dc.subjectHigh-throughput sequencingeng
dc.titleComparing microbiota profiles in induced and spontaneous sputum samples in COPD patientsen_US
dc.typePeer reviewed
dc.typeJournal article
dc.rights.holderCopyright 2017 The Author(s)
dc.source.journalRespiratory Research

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