Vis enkel innførsel

dc.contributor.authorMelve, Guro Kristinen_US
dc.contributor.authorErsvær, Elisabethen_US
dc.contributor.authorEide, Geir Egilen_US
dc.contributor.authorKristoffersen, Einar Klæboeen_US
dc.contributor.authorBruserud, Øysteinen_US
dc.PublishedMelve GK, Ersvær E, Eide GE, Kristoffersen EK, Bruserud Ø. Peripheral blood stem cell mobilization in healthy Donors by granulocyte colony-stimulating factor causes preferential mobilization of lymphocyte subsets. Frontiers in Immunology. 2018;9:845eng
dc.description.abstractBackground: Allogeneic hematopoietic stem cell transplantation is associated with a high risk of immune-mediated post-transplant complications. Graft depletion of immunocompetent cell subsets is regarded as a possible strategy to reduce this risk without reducing antileukemic immune reactivity. Study design and methods: We investigated the effect of hematopoietic stem cell mobilization with granulocyte colony-stimulating factor (G-CSF) on peripheral blood and stem cell graft levels of various T, B, and NK cell subsets in healthy donors. The results from flow cytometric cell quantification were examined by bioinformatics analyses. Results: The G-CSF-induced mobilization of lymphocytes was a non-random process with preferential mobilization of naïve CD4+ and CD8+ T cells together with T cell receptor αβ+ T cells, naïve T regulatory cells, type 1 T regulatory cells, mature and memory B cells, and cytokine-producing NK cells. Analysis of circulating lymphoid cell capacity to release various cytokines (IFNγ, IL10, TGFβ, IL4, IL9, IL17, and IL22) showed preferential mobilization of IL10 releasing CD4+ T cells and CD3−19− cells. During G-CSF treatment, the healthy donors formed two subsets with generally strong and weaker mobilization of immunocompetent cells, respectively; hence the donors differed in their G-CSF responsiveness with regard to mobilization of immunocompetent cells. The different responsiveness was not reflected in the graft levels of various immunocompetent cell subsets. Furthermore, differences in donor G-CSF responsiveness were associated with time until platelet engraftment. Finally, strong G-CSF-induced mobilization of various T cell subsets seemed to increase the risk of recipient acute graft versus host disease, and this was independent of the graft T cell levels. Conclusion: Healthy donors differ in their G-CSF responsiveness and preferential mobilization of immunocompetent cells. This difference seems to influence post-transplant recipient outcomes.en_US
dc.rightsAttribution CC BYeng
dc.subjectgraft versus host diseaseeng
dc.subjectgranulocyte colony-stimulating factoreng
dc.subjecthematopoietic stem cell mobilizationeng
dc.subjecthematopoietic stem cell transplantationeng
dc.subjectimmune reconstitutioneng
dc.subjectliving donorseng
dc.subjectperipheral blood stem cellseng
dc.titlePeripheral blood stem cell mobilization in healthy Donors by granulocyte colony-stimulating factor causes preferential mobilization of lymphocyte subsetsen_US
dc.typePeer reviewed
dc.typeJournal article
dc.rights.holderCopyright 2018 The Author(s)
dc.source.journalFrontiers in Immunology

Tilhørende fil(er)


Denne innførselen finnes i følgende samling(er)

Vis enkel innførsel

Attribution CC BY
Med mindre annet er angitt, så er denne innførselen lisensiert som Attribution CC BY