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dc.contributor.authorOftedal, Bergithe Eikelanden_US
dc.contributor.authorArdesjo Lundgren, Britaen_US
dc.contributor.authorHamm, Daviden_US
dc.contributor.authorGan, Poh-Yien_US
dc.contributor.authorHoldsworth, Stephen Ren_US
dc.contributor.authorHahn, Christopher N.en_US
dc.contributor.authorSchreiber, Andreas W.en_US
dc.contributor.authorScott, Hamish S.en_US
dc.date.accessioned2018-09-05T12:03:01Z
dc.date.available2018-09-05T12:03:01Z
dc.date.issued2017-07
dc.PublishedOftedal BE, Ardesjo Lundgren, Hamm D, Gan, Holdsworth, Hahn CN, Schreiber AW, Scott HS. T cell receptor assessment in autoimmune disease requires access to the most adjacent immunologically active organ. Journal of Autoimmunity. 2017;81:24-33eng
dc.identifier.issn1095-9157
dc.identifier.issn0896-8411
dc.identifier.urihttps://hdl.handle.net/1956/18405
dc.description.abstractNext generation sequencing of T and B cell receptors is emerging as a valuable and effective method to diagnose and monitor hematopoietic malignancies. So far, this approach has not been fully explored in regard to autoimmune diseases. T cells develop in the thymus where they undergo positive and negative selection, and the autoimmune regulator (Aire) is central in the establishment of immunological tolerance. Loss of Aire leads to severe multiorgan autoimmune disease with infiltration of autoreactive T cells in affected organs. Here, we have utilized next generation sequencing technology to investigate the T cell receptor repertoire in autoimmunity induced by immunization of mice with a self-antigen, myeloperoxidase. By investigating the T cell receptor repertoire in peripheral blood, spleen and lumbar lymph nodes from naïve and immunized Aire −/− mice and wild type littermates, changes in the usage of V and J genes were evident. Our results identify TCR clonotypes which could be potential targets for immune therapy. Also, Aire −/− autoimmunity is driven by a variety of autoantigens where the autoimmune response is highly polyclonal, and access to the most adjacent immunologically active tissue is required to identify T cell receptor sequences that are potentially unique to the antigen in Aire−/− immunized mice.en_US
dc.language.isoengeng
dc.publisherElseviereng
dc.rightsAttribution CC BY-NC-NDeng
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/eng
dc.subjectNext generation sequencingeng
dc.subjectT cell receptor repertoireeng
dc.subjectAutoimmune regulatoreng
dc.titleT cell receptor assessment in autoimmune disease requires access to the most adjacent immunologically active organen_US
dc.typePeer reviewed
dc.typeJournal article
dc.date.updated2018-03-05T14:24:33Z
dc.description.versionpublishedVersionen_US
dc.rights.holderCopyright 2017 The Author(s)
dc.identifier.doihttps://doi.org/10.1016/j.jaut.2017.03.002
dc.identifier.cristin1541830
dc.source.journalJournal of Autoimmunity


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