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dc.contributor.authorStrauss, Philippen_US
dc.contributor.authorMarti, Hans-Peteren_US
dc.contributor.authorBeisland, Christianen_US
dc.contributor.authorScherer, Andreasen_US
dc.contributor.authorLysne, Vegarden_US
dc.contributor.authorLeh, Sabine Mariaen_US
dc.contributor.authorFlatberg, Arnaren_US
dc.contributor.authorKoch, Even Evjenen_US
dc.contributor.authorBeisvag, Vidaren_US
dc.contributor.authorLandolt, Lea Zoeen_US
dc.contributor.authorSkogstrand, Trudeen_US
dc.contributor.authorEikrem, Øystein Solbergen_US
dc.date.accessioned2019-05-28T09:19:50Z
dc.date.available2019-05-28T09:19:50Z
dc.date.issued2018-03-10
dc.PublishedStrauss P, Marti HP, Beisland C, Scherer A, Lysne V, Leh S, Flatberg A, Koch EE, Beisvag V, Landolt LZ, Skogstrand T, Eikrem ØS. Expanding the utilization of formalin-fixed, paraffin-embedded archives: Feasibility of miR-Seq for disease exploration and biomarker development from biopsies with clear cell renal cell Carcinoma. International Journal of Molecular Sciences. 2018;19(3):803eng
dc.identifier.issn1422-0067
dc.identifier.urihttps://hdl.handle.net/1956/19759
dc.description.abstractNovel predictive tools for clear cell renal cell carcinoma (ccRCC) are urgently needed. MicroRNAs (miRNAs) have been increasingly investigated for their predictive value, and formalin-fixed paraffin-embedded biopsy archives may potentially be a valuable source of miRNA sequencing material, as they remain an underused resource. Core biopsies of both cancerous and adjacent normal tissues were obtained from patients (n = 12) undergoing nephrectomy. After small RNA-seq, several analyses were performed, including classifier evaluation, obesity-related inquiries, survival analysis using publicly available datasets, comparisons to the current literature and ingenuity pathway analyses. In a comparison of tumour vs. normal, 182 miRNAs were found with significant differential expression; miR-155 was of particular interest as it classified all ccRCC samples correctly and correlated well with tumour size (R2 = 0.83); miR-155 also predicted poor survival with hazard ratios of 2.58 and 1.81 in two different TCGA (The Cancer Genome Atlas) datasets in a univariate model. However, in a multivariate Cox regression analysis including age, sex, cancer stage and histological grade, miR-155 was not a statistically significant survival predictor. In conclusion, formalin-fixed paraffin-embedded biopsy tissues are a viable source of miRNA-sequencing material. Our results further support a role for miR-155 as a promising cancer classifier and potentially as a therapeutic target in ccRCC that merits further investigation.en_US
dc.language.isoengeng
dc.publisherMDPIeng
dc.rightsAttribution CC BYeng
dc.rights.urihttp://creativecommons.org/licenses/by/4.0eng
dc.subjectmicroRNA/miRNAeng
dc.subjectmiR-155eng
dc.subjectclear cell renal cell carcinoma/ccRCCeng
dc.subjectformalin-fixed paraffin-embedded/FFPEeng
dc.subjectnext generation sequencing/NGSeng
dc.titleExpanding the utilization of formalin-fixed, paraffin-embedded archives: Feasibility of miR-Seq for disease exploration and biomarker development from biopsies with clear cell renal cell Carcinomaen_US
dc.typePeer reviewed
dc.typeJournal article
dc.date.updated2019-01-16T13:36:58Z
dc.description.versionpublishedVersionen_US
dc.rights.holderCopyright 2018 The Author(s)
dc.identifier.doihttps://doi.org/10.3390/ijms19030803
dc.identifier.cristin1590269
dc.source.journalInternational Journal of Molecular Sciences


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