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dc.contributor.authorBrenner, Annetteen_US
dc.contributor.authorAasebø, Eliseen_US
dc.contributor.authorHernandez-Valladares, Mariaen_US
dc.contributor.authorSelheim, Frodeen_US
dc.contributor.authorBerven, Frodeen_US
dc.contributor.authorGrønningsæter, Ida Sofieen_US
dc.contributor.authorBartaula-Brevik, Sushmaen_US
dc.contributor.authorBruserud, Øysteinen_US
dc.date.accessioned2019-05-29T11:16:54Z
dc.date.available2019-05-29T11:16:54Z
dc.date.issued2019-01-10
dc.PublishedBrenner AK, Aasebø E, Hernandez-Valladares M, Selheim F, Berven F, Grønningsæter IS, Bartaula-Brevik S, Bruserud Ø. The Capacity of Long-Term In Vitro Proliferation of Acute Myeloid Leukemia Cells Supported Only by Exogenous Cytokines Is Associated with a Patient Subset with Adverse Outcome. Cancers. 2019;11(1):73eng
dc.identifier.issn2072-6694
dc.identifier.urihttps://hdl.handle.net/1956/19808
dc.description.abstractAcute myeloid leukemia (AML) is an aggressive malignancy, which is highly heterogeneous with regard to chemosensitivity and biological features. The AML cell population is organized in a hierarchy that is reflected in the in vitro growth characteristics, with only a minority of cells being able to proliferate for more than two weeks. In this study, we investigated the ability of AML stem cells to survive and proliferate in suspension cultures in the presence of exogenous mediators but without supporting non-leukemic cells. We saw that a high number of maintained stem cells (i.e., a large number of clonogenic cells after five weeks of culture) was associated with decreased overall survival for patients receiving intensive chemotherapy; this prognostic impact was also detected in the multivariate/adjusted analysis. Furthermore, the patients with many clonogenic cells presented more frequently with mutations in transcription-related genes, and also showed a higher abundance of proteins involved in transcription at the time of diagnosis. In conclusion, the growth characteristics of the long-term proliferating leukemic stem cells seem to have an independent prognostic impact in human AML, and these characteristics appear to be reflected by the mutational landscape and the proteome of the patients at the time of diagnosis.en_US
dc.language.isoengeng
dc.publisherMDPIeng
dc.rightsAttribution CC BYeng
dc.rights.urihttp://creativecommons.org/licenses/by/4.0eng
dc.subjectAcute myeloid leukemiaeng
dc.subjectclonogenic cellseng
dc.subjectcolony formationeng
dc.subjectCytokineseng
dc.subjectdifferentiationeng
dc.subjecttranscriptioneng
dc.titleThe Capacity of Long-Term In Vitro Proliferation of Acute Myeloid Leukemia Cells Supported Only by Exogenous Cytokines Is Associated with a Patient Subset with Adverse Outcomeen_US
dc.typePeer reviewed
dc.typeJournal article
dc.date.updated2019-01-24T10:30:50Z
dc.description.versionpublishedVersionen_US
dc.rights.holderCopyright 2019 The Author(s)
dc.identifier.doihttps://doi.org/10.3390/cancers11010073
dc.identifier.cristin1660576
dc.source.journalCancers


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