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dc.contributor.authorUtheim, Tor Paaskeen_US
dc.contributor.authorUtheim, Øygunn Aassen_US
dc.contributor.authorSalvanos, Panagiotisen_US
dc.contributor.authorJackson, Catherineen_US
dc.contributor.authorSchrader, Stefanen_US
dc.contributor.authorGeerling, Gerden_US
dc.contributor.authorSehic, Ameren_US
dc.PublishedUtheim TP, Utheim ØA, Salvanos P, Jackson C, Schrader, Geerling, Sehic A. Concise review: Altered versus unaltered amniotic membrane as a substrate for limbal epithelial cells. Stem Cells Translational Medicine. 2018;7(5):415-427eng
dc.description.abstractLimbal stem cell deficiency (LSCD) can result from a variety of corneal disorders, including chemical and thermal burns, infections, and autoimmune diseases. The symptoms of LSCD may include irritation, epiphora, blepharospasms, photophobia, pain, and decreased vision. There are a number of treatment options, ranging from nonsurgical treatments for mild LSCD to various forms of surgery that involve different cell types cultured on various substrates. Ex vivo expansion of limbal epithelial cells (LEC) involves the culture of LEC harvested either from the patient, a living relative, or a cadaver on a substrate in the laboratory. Following the transfer of the cultured cell sheet onto the cornea of patients suffering from LSCD, a successful outcome can be expected in approximately three out of four patients. The phenotype of the cultured cells has proven to be a key predictor of success. The choice of culture substrate is known to affect the phenotype. Several studies have shown that amniotic membrane (AM) can be used as a substrate for expansion of LEC for subsequent transplantation in the treatment of LSCD. There is currently a debate over whether AM should be denuded (i.e., de‐epithelialized) prior to LEC culture, or whether this substrate should remain intact. In addition, crosslinking of the AM has been used to increase the thermal and mechanical stability, optical transparency, and resistance to collagenase digestion of AM. In the present review, we discuss the rationale for using altered versus unaltered AM as a culture substrate for LEC.en_US
dc.publisherWiley Open Accesseng
dc.rightsAttribution CC BY-NC-NDeng
dc.subjectCell biologyeng
dc.subjectGene expressioneng
dc.subjectCell transplantationeng
dc.titleConcise review: Altered versus unaltered amniotic membrane as a substrate for limbal epithelial cellsen_US
dc.typePeer reviewed
dc.typeJournal article
dc.rights.holderCopyright 2018 The Authors
dc.source.journalStem Cells Translational Medicine

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