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dc.contributor.authorOsuagwu, Nelson Uchechukwuen_US
dc.contributor.authorDölle, Christianen_US
dc.contributor.authorTzoulis, Charalamposen_US
dc.date.accessioned2020-03-16T10:18:50Z
dc.date.available2020-03-16T10:18:50Z
dc.date.issued2019
dc.PublishedOsuagwu N, Dölle C, Tzoulis C. Poly-ADP-ribose assisted protein localization resolves that DJ-1, but not LRRK2 or α-synuclein, is localized to the mitochondrial matrix. PLOS ONE. 2019;14:e0219909eng
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/1956/21505
dc.description.abstractSeveral proteins linked to familial Parkinson disease have been associated with mitochondrial (dys-)function and have been described to reside within mitochondria. The putative mitochondrial and sub-mitochondrial localization of these proteins remains disputed, however, potentially due to conflicting results obtained by diverging technical approaches. Using the high-resolution poly-ADP-ribose assisted protein localization assay that also allows for detection of low level and even partial mitochondrial matrix localization, we demonstrate here that DJ-1, but not LRRK2 or α-synuclein, resides in the mitochondrial matrix. The localization of the proteins was not changed in cellular stress models of Parkinson disease and, in case of α-synuclein, not affected by pathological mutations. Our results verify the ability of DJ-1 to carry out its role also from within mitochondria and suggest that LRRK2 and α-synuclein may interact with and affect mitochondria from outside the mitochondrial matrix.en_US
dc.language.isoengeng
dc.publisherPLOSeng
dc.rightsAttribution CC BYeng
dc.rights.urihttp://creativecommons.org/licenses/by/4.0eng
dc.titlePoly-ADP-ribose assisted protein localization resolves that DJ-1, but not LRRK2 or α-synuclein, is localized to the mitochondrial matrixen_US
dc.typePeer reviewed
dc.typeJournal article
dc.date.updated2019-12-13T11:55:56Z
dc.description.versionpublishedVersionen_US
dc.rights.holderCopyright 2019 The Author(s)
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0219909
dc.identifier.cristin1712792
dc.source.journalPLoS ONE


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