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dc.contributor.authorMyklebust, Mette Pernilleen_US
dc.contributor.authorRosenlund, Benedicteen_US
dc.contributor.authorGjengstø, Pederen_US
dc.contributor.authorBercea, Bogdan Stefanen_US
dc.contributor.authorKarlsdottir, Åsaen_US
dc.contributor.authorBrydøy, Marianneen_US
dc.contributor.authorDahl, Olaven_US
dc.date.accessioned2020-06-08T13:37:46Z
dc.date.available2020-06-08T13:37:46Z
dc.date.issued2019-05-22
dc.PublishedMyklebust MP, Rosenlund B, Gjengstø P, Bercea, Karlsdottir Á, Brydøy M, Dahl O. Quantitative PCR Measurement of miR-371a-3p and miR-372-p Is Influenced by Hemolysis. Frontiers in Genetics. 2019;10:463(MAY)eng
dc.identifier.issn1664-8021
dc.identifier.urihttps://hdl.handle.net/1956/22493
dc.description.abstractCell-free microRNAs have been reported as biomarkers for several diseases. For testicular germ cell tumors (GCT), circulating microRNAs 371a-3p and 372-3p in serum and plasma have been proposed as biomarkers for diagnostic and disease monitoring purposes. The most widely used method for quantification of specific microRNAs in serum and plasma is reverse transcriptase real-time quantitative PCR (RT-qPCR) by the comparative Ct-method. In this method one or several reference genes or reference microRNAs are needed in order to normalize and calculate the relative microRNA levels across samples. One of the pitfalls in analysis of microRNAs from serum and plasma is the release of microRNAs from blood cells during hemolysis. This is an important issue because varying degrees of hemolysis are not uncommon in routine blood sampling. Thus, hemolysis must be taken into consideration when working with circulating microRNAs from blood. miR-93-5p, miR-30b-5p, and miR-20a-5p have been reported as reference microRNA in analysis of the miR-371a-373 cluster. We here show how these three microRNAs are influenced by hemolysis. We also propose a new reference microRNA, miR-191-5p, which is relatively stable in serum samples with mild hemolysis. In addition, we show how hemolysis can have effect on the reported microRNA levels in patient samples when these reference microRNAs are used in samples with varying levels of hemolysis.en_US
dc.language.isoengeng
dc.publisherFrontierseng
dc.rightsAttribution CC BYeng
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/eng
dc.titleQuantitative PCR Measurement of miR-371a-3p and miR-372-p Is Influenced by Hemolysisen_US
dc.typePeer reviewed
dc.typeJournal article
dc.date.updated2020-02-10T08:24:34Z
dc.description.versionpublishedVersionen_US
dc.rights.holderCopyright 2019 Myklebust, Rosenlund, Gjengstø, Bercea, Karlsdottir, Brydøy and Dahl.
dc.identifier.doihttps://doi.org/10.3389/fgene.2019.00463
dc.identifier.cristin1720991
dc.source.journalFrontiers in Genetics


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