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dc.contributor.authorTeodora, Paunas Flaviaen_US
dc.contributor.authorFinne, Kennethen_US
dc.contributor.authorLeh, Sabine Mariaen_US
dc.contributor.authorOsman, Tarig Al-Hadien_US
dc.contributor.authorMarti, Hans-Peteren_US
dc.contributor.authorBerven, Frodeen_US
dc.contributor.authorVikse, Bjørn Egilen_US
dc.PublishedTeodora PF, Finne K, Leh S, Osman T, Marti HP, Berven F, Vikse BE. Characterization of glomerular extracellular matrix in IgA nephropathy by proteomic analysis of laser-captured microdissected glomeruli. BMC Nephrology. 2019;20:410eng
dc.description.abstractBackground IgA nephropathy (IgAN) involves mesangial matrix expansion, but the proteomic composition of this matrix is unknown. The present study aimed to characterize changes in extracellular matrix in IgAN. Methods In the present study we used mass spectrometry-based proteomics in order to quantitatively compare protein abundance between glomeruli of patients with IgAN (n = 25) and controls with normal biopsy findings (n = 15). Results Using a previously published paper by Lennon et al. and cross-referencing with the Matrisome database we identified 179 extracellular matrix proteins. In the comparison between IgAN and controls, IgAN glomeruli showed significantly higher abundance of extracellular matrix structural proteins (e.g periostin, vitronectin, and extracellular matrix protein 1) and extracellular matrix associated proteins (e.g. azurocidin, myeloperoxidase, neutrophil elastase, matrix metalloproteinase-9 and matrix metalloproteinase 2). Periostin (fold change 3.3) and azurocidin (3.0) had the strongest fold change between IgAN and controls; periostin was also higher in IgAN patients who progressed to ESRD as compared to patients who did not. Conclusion IgAN is associated with widespread changes of the glomerular extracellular matrix proteome. Proteins important in glomerular sclerosis or inflammation seem to be most strongly increased and periostin might be an important marker of glomerular damage in IgAN.en_US
dc.rightsAttribution CC BYeng
dc.subjectIgA nephropathyeng
dc.titleCharacterization of glomerular extracellular matrix in IgA nephropathy by proteomic analysis of laser-captured microdissected glomerulien_US
dc.typePeer reviewed
dc.typeJournal article
dc.rights.holderCopyright 2019 The Author(s)
dc.source.journalBMC Nephrology

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