Vis enkel innførsel

dc.contributor.authorRaasakka, Arneen_US
dc.contributor.authorKursula, Petrien_US
dc.date.accessioned2020-06-08T16:58:40Z
dc.date.available2020-06-08T16:58:40Z
dc.date.issued2019-09-23
dc.PublishedRaasakka A, Kursula P. Stability and flexibility of full-length human oligodendrocytic QKI6. BMC Research Notes. 2019;12:609eng
dc.identifier.issn1756-0500
dc.identifier.urihttps://hdl.handle.net/1956/22508
dc.description.abstractObjective: Oligodendrocytes account for myelination in the central nervous system. During myelin compaction, key proteins are translated in the vicinity of the myelin membrane, requiring targeted mRNA transport. Quaking isoform 6 (QKI6) is a STAR domain-containing RNA transport protein, which binds a conserved motif in the 3′-UTR of certain mRNAs, affecting the translation of myelination-involved proteins. RNA binding has been earlier structurally characterized, but information about full-length QKI6 conformation is lacking. Based on known domains and structure predicitons, we expected full-length QKI6 to be flexible and carry disordered regions. Hence, we carried out biophysical and structural characterization of human QKI6. Results: We expressed and purified full-length QKI6 and characterized it using mass spectrometry, light scattering, small-angle X-ray scattering, and circular dichroism spectroscopy. QKI6 was monodisperse, folded, and mostly dimeric, being oxidation-sensitive. The C-terminal tail was intrinsically disordered, as predicted. In the absence of RNA, the RNA-binding subdomain is likely to present major flexibility. In thermal stability assays, a double sequential unfolding behaviour was observed in the presence of phosphate, which may interact with the RNA-binding domain. The results confirm the flexibility and partial disorder of QKI6, which may be functionally relevant.en_US
dc.language.isoengeng
dc.publisherBMCeng
dc.rightsAttribution CC BYeng
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/eng
dc.subjectMyelin sheatheng
dc.subjectProtein stabilityeng
dc.subjectRNA-binding proteineng
dc.subjectCircular dichroismeng
dc.subjectSAXSeng
dc.titleStability and flexibility of full-length human oligodendrocytic QKI6en_US
dc.typePeer reviewed
dc.typeJournal article
dc.date.updated2020-01-17T12:29:31Z
dc.description.versionpublishedVersionen_US
dc.rights.holderCopyright 2019 The Author(s)
dc.identifier.doihttps://doi.org/10.1186/s13104-019-4629-x
dc.identifier.cristin1746014
dc.source.journalBMC Research Notes


Tilhørende fil(er)

Thumbnail

Denne innførselen finnes i følgende samling(er)

Vis enkel innførsel

Attribution CC BY
Med mindre annet er angitt, så er denne innførselen lisensiert som Attribution CC BY