Show simple item record

dc.contributor.authorMazzawi, Tareken_US
dc.contributor.authorHausken, Trygveen_US
dc.contributor.authorHov, Johannes Espolin Roksunden_US
dc.contributor.authorValeur, Jørgenen_US
dc.contributor.authorSangnes, Dag Andréen_US
dc.contributor.authorEl-Salhy, Magdyen_US
dc.contributor.authorGilja, Odd Helgeen_US
dc.contributor.authorHatlebakk, Jan Gunnaren_US
dc.contributor.authorLied, Gülen Arslanen_US
dc.date.accessioned2020-06-17T09:10:56Z
dc.date.available2020-06-17T09:10:56Z
dc.date.issued2019
dc.PublishedMazzawi T, Hausken T, Hov JR, Valeur J, Sangnes DA, El-Salhy M, Gilja OH, Hatlebakk JG, Lied G. Clinical response to fecal microbiota transplantation in patients with diarrhea-predominant irritable bowel syndrome is associated with normalization of fecal microbiota composition and short-chain fatty acid levels. Scandinavian Journal of Gastroenterology. 2019;54(6):690-699eng
dc.identifier.issn0036-5521
dc.identifier.issn1502-7708
dc.identifier.urihttps://hdl.handle.net/1956/22673
dc.description.abstractObjectives: Irritable bowel syndrome (IBS) may be associated with disturbances in gut microbiota composition and functions. We recently performed a study of fecal microbiota transplantation (FMT) in diarrhea-predominant IBS (IBS-D) and found that IBS symptoms improved and the gut microbiota profile changed following FMT. We now aimed to explore the effects of FMT on the gut microenvironment in further detail by using 16S rRNA sequencing for more extended microbiota profiling and analyzing bacterial fermentation products (SCFAs: short chain fatty acids). Materials and methods: The study included 13 patients (four females and nine males) with IBS-D according to Rome III criteria and 13 healthy donors. Freshly donated feces were administered into duodenum via gastroscopy. The patients completed symptom and quality of life (QoL) questionnaires and delivered feces before and 1, 3, 12 and 20/28 weeks after FMT. Microbiota analysis was performed by sequencing 16S rRNA gene with Illumina Miseq technology. Fecal concentrations of SCFAs were analyzed by vacuum distillation followed by gas chromatography. Results: Several gut microbiota taxa and SCFAs were significantly different in the patients at baseline compared to their donors. These differences normalized by the third week following FMT in parallel with significant improvement in symptoms and QoL. Responders had different gut microbiota profile and SCFAs than nonresponders. Significant correlations were found between the gut microenvironment and IBS symptoms. No adverse effects were reported. Conclusions: FMT restores alterations of the gut microenvironment in IBS-D patients during the first 3 weeks and improves their symptoms for up to 28 weeks.en_US
dc.language.isoengeng
dc.publisherTaylor & Franciseng
dc.titleClinical response to fecal microbiota transplantation in patients with diarrhea-predominant irritable bowel syndrome is associated with normalization of fecal microbiota composition and short-chain fatty acid levelsen_US
dc.typePeer reviewed
dc.typeJournal article
dc.date.updated2020-01-16T07:17:35Z
dc.description.versionacceptedVersionen_US
dc.rights.holderCopyright 2019 Informa UK Limited, trading as Taylor & Francis Group
dc.identifier.doihttps://doi.org/10.1080/00365521.2019.1624815
dc.identifier.cristin1705123
dc.source.journalScandinavian Journal of Gastroenterology


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record