Vis enkel innførsel

dc.contributor.authorVorobyeva, Anzhelika G.en_US
dc.contributor.authorStanton, Michaelen_US
dc.contributor.authorGodinat, Aurélienen_US
dc.contributor.authorLund, Kjetil B.en_US
dc.contributor.authorKarateev, Grigory G.en_US
dc.contributor.authorFrancis, Kevin P.en_US
dc.contributor.authorAllen, Elizabethen_US
dc.contributor.authorGelovani, Juri G.en_US
dc.contributor.authorMcCormack, Emmeten_US
dc.contributor.authorTangney, Marken_US
dc.contributor.authorDubikovskaya, Elena A.en_US
dc.date.accessioned2020-06-26T08:17:16Z
dc.date.available2020-06-26T08:17:16Z
dc.date.issued2015-06-25
dc.PublishedVorobyeva, Stanton, Godinat, Lund KB, Karateev, Francis, Allen E, Gelovani, McCormack E, Tangney, Dubikovskaya. Development of a bioluminescent nitroreductase probe for preclinical imaging. PLOS ONE. 2015;10(6):e0131037eng
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/1956/23028
dc.description.abstractBacterial nitroreductases (NTRs) have been widely utilized in the development of novel antibiotics, degradation of pollutants, and gene-directed enzyme prodrug therapy (GDEPT) of cancer that reached clinical trials. In case of GDEPT, since NTR is not naturally present in mammalian cells, the prodrug is activated selectively in NTR-transformed cancer cells, allowing high efficiency treatment of tumors. Currently, no bioluminescent probes exist for sensitive, non-invasive imaging of NTR expression. We therefore developed a "NTR caged luciferin" (NCL) probe that is selectively reduced by NTR, producing light proportional to the NTR activity. Here we report successful application of this probe for imaging of NTR in vitro, in bacteria and cancer cells, as well as in vivo in mouse models of bacterial infection and NTR-expressing tumor xenografts. This novel tool should significantly accelerate the development of cancer therapy approaches based on GDEPT and other fields where NTR expression is important.en_US
dc.language.isoengeng
dc.publisherPLoSeng
dc.rightsAttribution CC BYeng
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/eng
dc.titleDevelopment of a bioluminescent nitroreductase probe for preclinical imagingen_US
dc.typePeer reviewed
dc.typeJournal article
dc.date.updated2020-02-05T10:48:20Z
dc.description.versionpublishedVersionen_US
dc.rights.holderCopyright 2015 Vorobyeva et al.
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0131037
dc.identifier.cristin1275481
dc.source.journalPLoS ONE


Tilhørende fil(er)

Thumbnail

Denne innførselen finnes i følgende samling(er)

Vis enkel innførsel

Attribution CC BY
Med mindre annet er angitt, så er denne innførselen lisensiert som Attribution CC BY