dc.contributor.author | Stepnov, Anton | |
dc.contributor.author | Fredriksen, Lasse | |
dc.contributor.author | Steen, Ida Helene | |
dc.contributor.author | Stokke, Runar | |
dc.contributor.author | Eijsink, Vincent | |
dc.date.accessioned | 2020-08-07T12:27:25Z | |
dc.date.available | 2020-08-07T12:27:25Z | |
dc.date.issued | 2019-09-06 | |
dc.Published | Stepnov, Fredriksen, Steen, Stokke, Eijsink. Identification and characterization of a hyperthermophilic GH9 cellulase from the Arctic Mid-Ocean Ridge vent field. PLOS ONE. 2019;14(9):e0222216 | eng |
dc.identifier.issn | 1932-6203 | en_US |
dc.identifier.uri | https://hdl.handle.net/1956/23567 | |
dc.description.abstract | A novel GH9 cellulase (AMOR_GH9A) was discovered by sequence-based mining of a unique metagenomic dataset collected at the Jan Mayen hydrothermal vent field. AMOR_GH9A comprises a signal peptide, a catalytic domain and a CBM3 cellulose-binding module. AMOR_GH9A is an exceptionally stable enzyme with a temperature optimum around 100°C and an apparent melting temperature of 105°C. The novel cellulase retains 64% of its activity after 4 hours of incubation at 95°C. The closest characterized homolog of AMOR_GH9A is TfCel9A, a processive endocellulase from the model thermophilic bacterium Thermobifida fusca (64.2% sequence identity). Direct comparison of AMOR_GH9A and TfCel9A revealed that AMOR_GH9A possesses higher activity on soluble and amorphous substrates (phosphoric acid swollen cellulose, konjac glucomannan) and has an ability to hydrolyse xylan that is lacking in TfCel9A. | en_US |
dc.language.iso | eng | eng |
dc.publisher | PLoS | en_US |
dc.rights | Attribution CC BY | eng |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | eng |
dc.title | Identification and characterization of a hyperthermophilic GH9 cellulase from the Arctic Mid-Ocean Ridge vent field | en_US |
dc.type | Peer reviewed | |
dc.type | Journal article | |
dc.date.updated | 2020-01-02T14:21:17Z | |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | Copyright 2019 The Authors | en_US |
dc.identifier.doi | https://doi.org/10.1371/journal.pone.0222216 | |
dc.identifier.cristin | 1728196 | |
dc.source.journal | PLOS ONE | |
dc.relation.project | Norges forskningsråd: 270038 | |
dc.relation.project | Norges forskningsråd: 179560 | |
dc.relation.project | Norges forskningsråd: 226247 | |