dc.contributor.author | Lam, Margaret M.C. | en_US |
dc.contributor.author | Wyres, Kelly L. | en_US |
dc.contributor.author | Wick, Ryan R. | en_US |
dc.contributor.author | Judd, Louise M. | en_US |
dc.contributor.author | Fostervold, Aasmund | en_US |
dc.contributor.author | Holt, Kathryn E. | en_US |
dc.contributor.author | Löhr, Iren Høyland | en_US |
dc.date.accessioned | 2020-08-14T12:31:18Z | |
dc.date.available | 2020-08-14T12:31:18Z | |
dc.date.issued | 2019 | |
dc.Published | Lam MMC, Wyres KL, Wick RR, Judd LM, Fostervold A, Holt KE, Löhr IH. Convergence of virulence and MDR in a single plasmid vector in MDR Klebsiella pneumoniae ST15. Journal of Antimicrobial Chemotherapy. 2019;74(5):1218-1222 | eng |
dc.identifier.issn | 0305-7453 | |
dc.identifier.issn | 1460-2091 | |
dc.identifier.uri | https://hdl.handle.net/1956/23784 | |
dc.description.abstract | Background: MDR and hypervirulence (hv) are typically observed in separate Klebsiella pneumoniae populations. However, convergent strains with both properties have been documented and potentially pose a high risk to public health in the form of invasive infections with limited treatment options. Objectives: Our aim was to characterize the genetic determinants of virulence and antimicrobial resistance (AMR) in two ESBL-producing K. pneumoniae isolates belonging to the international MDR clone ST15. Methods: The complete genome sequences of both isolates, including their plasmids, were resolved using Illumina and Oxford Nanopore sequencing. Results: Both isolates carried large mosaic plasmids in which AMR and virulence loci have converged within the same vector. These closely related mosaic hv-MDR plasmids include sequences typical of the K. pneumoniae virulence plasmid 1 (KpVP-1; including aerobactin synthesis locus iuc) fused with sequences typical of IncFIIK conjugative AMR plasmids. One hv-MDR plasmid carried three MDR elements encoding the ESBL gene blaCTX-M-15 and seven other AMR genes (blaTEM, aac3'-IIa, dfrA1, satA2, blaSHV, sul1 and aadA1). The other carried remnants of these elements encoding blaTEM and aac3'-IIa, and blaCTX-M-15 was located in a second plasmid in this isolate. The two isolates originated from patients hospitalized in Norway but have epidemiological and genomic links to Romania. Conclusions: The presence of both virulence and AMR determinants on a single vector enables simultaneous transfer in a single event and potentially rapid emergence of hv-MDR K. pneumoniae clones. This highlights the importance of monitoring for such convergence events with stringent genomic surveillance. | en_US |
dc.language.iso | eng | eng |
dc.publisher | Oxford University Press | eng |
dc.rights | Attribution-NonCommercial CC BY-NC | eng |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/4.0/ | eng |
dc.title | Convergence of virulence and MDR in a single plasmid vector in MDR Klebsiella pneumoniae ST15 | en_US |
dc.type | Peer reviewed | |
dc.type | Journal article | |
dc.date.updated | 2020-01-30T13:09:59Z | |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | Copyright 2019 The Author(s) | |
dc.identifier.doi | https://doi.org/10.1093/jac/dkz028 | |
dc.identifier.cristin | 1749633 | |
dc.source.journal | Journal of Antimicrobial Chemotherapy | |