dc.contributor.author | Narrevik, Tommy | eng |
dc.date.accessioned | 2009-07-15T13:24:25Z | |
dc.date.available | 2009-07-15T13:24:25Z | |
dc.date.issued | 2008-11-27 | eng |
dc.date.submitted | 2008-11-27 | eng |
dc.identifier.uri | https://hdl.handle.net/1956/3360 | |
dc.description.abstract | Mass spectrometry(MS) have become an increasingly popular analysis method for high throughput experiments on proteins in biology. SILAC(stable isotope labeling by amino acids in cell culture) is a method within MS that uses labels to differentiate between the same protein from two cell cultures(protein pairs) of the same organism. To identify the protein pairs in SILAC MS/MS is used. The current/automatic method for MS/MS uses the most intense peaks from each single MS for the MS/MS analysis. This often result in incomplete pairs, and proteins that have a low abundance gets ignored. We created a program able to identify these pairs, and the researcher can use the data from it as an inclusion list for the MS/MS run. The program should be easy to maintain and use for other MS data analysis. | en_US |
dc.format.extent | 2723301 bytes | eng |
dc.format.mimetype | application/pdf | eng |
dc.language.iso | eng | eng |
dc.publisher | The University of Bergen | en_US |
dc.subject | SILAC | eng |
dc.subject | MS | eng |
dc.subject | MS/MS | eng |
dc.subject | Labeled | eng |
dc.title | MassAnalyzer, a program to help find labeled peptides and compare them to their unlabeled counterparts in a SILAC experiment | en_US |
dc.type | Master thesis | |
dc.rights.holder | The author | en_US |
dc.rights.holder | Copyright the author. All rights reserved | en_US |
dc.description.degree | Master i Informatikk - bioinformatikk | en_US |
dc.description.localcode | MAMN-INFBI | |
dc.description.localcode | INFBI | |
dc.subject.nus | 754115 | eng |
dc.subject.nsi | VDP::Teknologi: 500::Bioteknologi: 590 | nob |
fs.subjectcode | INFBI | |