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dc.contributor.authorStavrum, Rutheng
dc.contributor.authorStavrum, Anne-Kristineng
dc.contributor.authorValvatne, Håvardeng
dc.contributor.authorRiley, Lee W.eng
dc.contributor.authorUlvestad, Ellingeng
dc.contributor.authorJonassen, Ingeeng
dc.contributor.authorAssmus, Jørgeng
dc.contributor.authorDoherty, Tanya Markeng
dc.contributor.authorGrewal, Harleen M. S.eng
dc.PublishedPLoS ONE 6(10): e26295en
dc.description.abstractThe outcome of many infections depends on the initial interactions between agent and host. Aiming at elucidating the effect of the M. tuberculosis Mce1 protein complex on host transcriptional and immunological responses to infection with M. tuberculosis, RNA from murine macrophages at 15, 30, 60 min, 4 and 10 hrs post-infection with M. tuberculosis H37Rv or Dmce1 H37Rv was analyzed by whole-genome microarrays and RT-QPCR. Immunological responses were measured using a 23-plex cytokine assay. Compared to uninfected controls, 524 versus 64 genes were up-regulated by 15 min post H37Rvand D-mce1 H37Rv-infection, respectively. By 15 min post-H37Rv infection, a decline of 17 cytokines combined with upregulation of Ccl24 (26.5-fold), Clec4a2 (23.2-fold) and Pparc (10.5-fold) indicated an anti-inflammatory response initiated by IL-13. Down-regulation of Il13ra1 combined with up-regulation of Il12b (30.2-fold), suggested switch to a pro-inflammatory response by 4 hrs post H37Rv-infection. Whereas no significant change in cytokine concentration or transcription was observed during the first hour post D-mce1 H37Rv-infection, a significant decline of IL-1b, IL-9, IL-13, Eotaxin and GM-CSF combined with increased transcription of Il12b (25.1-fold) and Inb1 (17.9-fold) by 4 hrs, indicated a pro-inflammatory response. The balance between pro-and anti-inflammatory responses during the early stages of infection may have significant bearing on outcome.en_US
dc.publisherPublic Library of Scienceen_US
dc.rightsAttribution CC BYeng
dc.titleModulation of Transcriptional and Inflammatory Responses in Murine Macrophages by the Mycobacterium tuberculosis Mammalian Cell Entry (Mce) 1 Complexen_US
dc.typePeer reviewed
dc.typeJournal article
dc.rights.holderCopyright 2011 Stavrum et al.en_US
dc.subject.nsiVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710::Medical microbiology: 715en_US

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