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dc.contributor.authorDabija-Wolter, Gabrielaen_US
dc.contributor.authorBakken, Vidaren_US
dc.contributor.authorCimpan, Mihaela R.en_US
dc.contributor.authorJohannessen, Anne Christineen_US
dc.contributor.authorCostea, Daniela Elenaen_US
dc.date.accessioned2012-12-10T12:41:54Z
dc.date.available2012-12-10T12:41:54Z
dc.date.issued2012eng
dc.PublishedJournal of Oral Pathology & Medicineeng
dc.identifier.issn1600-0714 (online)
dc.identifier.urihttps://hdl.handle.net/1956/6222
dc.description.abstractBackground: The aim of this study was to develop and characterize standardized in vitro three-dimensional organotypic models of human junctional epithelium (JE) and sulcular epithelium (SE). Methods Organotypic models were constructed by growing human normal gingival keratinocytes on top of collagen matrices populated with gingival fibroblasts (GF) or periodontal ligament fibroblasts (PLF). Tissues obtained were harvested at different time points and assessed for epithelial morphology, proliferation (Ki67), expression of JE-specific markers (ODAM and FDC-SP), cytokeratins (CK), transglutaminase, filaggrin, and basement membrane proteins (collagen IV and laminin1). Results: The epithelial component in 3- and 5-day organotypics showed limited differentiation and expressed Ki-67, ODAM, FDC-SP, CK 8, 13, 16, 19, and transglutaminase in a similar fashion to control JE samples. PLF supported better than GF expression of CK19 and suprabasal proliferation, although statistically significant only at day 5. Basement membrane proteins started to be deposited only from day 5. The rate of proliferating cells as well as the percentage of CK19-expressing cells decreased significantly in 7- and 9-day cultures. Day 7 organotypics presented higher number of epithelial cell layers, proliferating cells in suprabasal layers, and CK expression pattern similar to SE. Conclusion: Both time in culture and fibroblast type had impact on epithelial phenotype. Five-day cultures with PLF are suggested as JE models, 7-day cultures with PLF or GF as SE models, while 9-day cultures with GF as gingival epithelium (GE) models. Such standard, reproducible models represent useful tools to study periodontal bacteria–host interactions in vitro.en_US
dc.language.isoengeng
dc.publisherJohn Wiley & Sons Ltd.eng
dc.relation.ispartof<a href="http://hdl.handle.net/1956/6223" target="blank">Interactions between Fusobacterium nucleatum and Primary Human Oral Cells: In Vitro Studies in Monolayer and Organotypic Culture Models</a>eng
dc.subjectOrganotypiceng
dc.subjectperiodontal tissueeng
dc.subjectcytokeratinseng
dc.subjectDifferentiationeng
dc.subjectoral epitheliaeng
dc.titleIn vitro reconstruction of human junctional and sulcular epitheliumen_US
dc.typeJournal article
dc.description.versiondraften_US
dc.rights.holderCopyright 2012 John Wiley & Sons A/S. All rights reserved
dc.identifier.doihttps://doi.org/10.1111/jop.12005
dc.identifier.cristin943101


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