dc.contributor.author | Sprater, Florian | en_US |
dc.contributor.author | Hovden, Arnt-Ove | en_US |
dc.contributor.author | Appel, Silke | en_US |
dc.date.accessioned | 2013-04-12T11:59:03Z | |
dc.date.available | 2013-04-12T11:59:03Z | |
dc.date.issued | 2012-11-19 | eng |
dc.Published | PLoS ONE 7(11): e49577 | eng |
dc.identifier.issn | 1932-6203 | |
dc.identifier.uri | https://hdl.handle.net/1956/6515 | |
dc.description.abstract | Dendritic cells (DC) are the only hematopoietic cells expressing the epithelial specific Ets transcription factor ESE-3. Here we analyzed presence and quantity of isoforms ESE-3a, ESE-3b and ESE-3j in various immunogenic and tolerogenic human monocyte-derived DC (moDC) and blood DC populations using quantitative real time PCR and immunoblot analyses. ESE-3a and ESE-3b were detectable in all moDC populations with ESE-3b being the main transcript. ESE-3b expression was upregulated in immunogenic moDC and downregulated in tolerogenic moDC compared to immature moDC. ESE-3a had similar transcript levels in immature and immunogenic moDC and had very low levels in tolerogenic moDC. In blood DC populations only splice variant ESE-3b was detectable. ESE-3j was not detectable in any of the DC populations. These findings suggest that ESE-3b is the functionally most important ESE-3 isoform in DC. | en_US |
dc.language.iso | eng | eng |
dc.publisher | Public Library of Science | eng |
dc.rights | Attribution CC BY | eng |
dc.rights.uri | http://creativecommons.org/licenses/by/2.5/ | eng |
dc.title | Expression of ESE-3 Isoforms in Immunogenic and Tolerogenic Human Monocyte-Derived Dendritic Cells | en_US |
dc.type | Peer reviewed | |
dc.type | Journal article | |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | Copyright 2012 Sprater et al. | |
dc.identifier.doi | https://doi.org/10.1371/journal.pone.0049577 | |
dc.identifier.cristin | 995882 | |