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dc.contributor.authorSoler, Ericeng
dc.contributor.authorAndrieu-Soler, Charlotteeng
dc.contributor.authorBoer, Ernie deeng
dc.contributor.authorBryne, Jan Christianeng
dc.contributor.authorThongjuea, Supateng
dc.contributor.authorStadhouders, Ralpheng
dc.contributor.authorPalstra, Robert-Janeng
dc.contributor.authorStevens, Maryeng
dc.contributor.authorKockx, Christeleng
dc.contributor.authorIJcken, Wilfred vaneng
dc.contributor.authorHou, Juneng
dc.contributor.authorSteinhoff, Christineeng
dc.contributor.authorRijkers, Erikjaneng
dc.contributor.authorLenhard, Boriseng
dc.contributor.authorGrosveld, Frankeng
dc.date.accessioned2014-03-14T11:37:22Z
dc.date.available2014-03-14T11:37:22Z
dc.date.issued2009eng
dc.identifier.issn0890-9369/10en_US
dc.identifier.urihttps://hdl.handle.net/1956/7851
dc.descriptionSupplemental material is available at <a href="http://www.genesdev.org" target="blank">http://www.genesdev.org</a>en_US
dc.description.abstractOne of the complexes formed by the hematopoietic transcription factor Gata1 is a complex with the Ldb1 (LIM domain-binding protein 1) and Tal1 proteins. It is known to be important for the development and differentiation of the erythroid cell lineage and is thought to be implicated in long-range interactions. Here, the dynamics of the composition of the complex—in particular, the binding of the negative regulators Eto2 and Mtgr1—are studied, in the context of their genome-wide targets. This shows that the complex acts almost exclusively as an activator, binding a very specific combination of sequences, with a positioning relative to transcription start site, depending on the type of the core promoter. The activation is accompanied by a net decrease in the relative binding of Eto2 and Mtgr1. A Chromosome Conformation Capture sequencing (3C-seq) assay also shows that the binding of the Ldb1 complex marks genomic interaction sites in vivo. This establishes the Ldb1 complex as a positive regulator of the final steps of erythroid differentiation that acts through the shedding of negative regulators and the active interaction between regulatory sequences.en_US
dc.language.isoengeng
dc.publisherCold Spring Harbor Laboratory Pressen_US
dc.relation.ispartof<a href="http://hdl.handle.net/1956/7852" target="blank">Analysis of transcription factor complexes and their associated chromatin interactions during erythropoiesis</a>en_US
dc.rightsAttribution-NonCommercial CC BY-NCeng
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/eng
dc.subjectChIP sequencingeng
dc.subjecttranscription factor complexeseng
dc.subjectDevelopmenteng
dc.subjectDifferentiationeng
dc.subjecterythropoiesiseng
dc.subjectlong-range interactionseng
dc.titleThe genome-wide dynamics of the binding of Ldb1 complexes during erythroid differentiationen_US
dc.typePeer reviewed
dc.typeJournal article
dc.description.versionpublishedVersionen_US
dc.rights.holderCopyright 2010 by Cold Spring Harbor Laboratory Press. Freely available online through the Genes & Development Open Access option. Distributed under the Creative Commons Attribution-Non-Commercial 4.0 International License (CC-BY-NC),en_US
dc.identifier.doihttps://doi.org/10.1101/gad.551810
dc.source.journalGenes & Development
dc.source.4024
dc.source.pagenumber277-289


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