dc.contributor.author | Soler, Eric | eng |
dc.contributor.author | Andrieu-Soler, Charlotte | eng |
dc.contributor.author | Boer, Ernie de | eng |
dc.contributor.author | Bryne, Jan Christian | eng |
dc.contributor.author | Thongjuea, Supat | eng |
dc.contributor.author | Stadhouders, Ralph | eng |
dc.contributor.author | Palstra, Robert-Jan | eng |
dc.contributor.author | Stevens, Mary | eng |
dc.contributor.author | Kockx, Christel | eng |
dc.contributor.author | IJcken, Wilfred van | eng |
dc.contributor.author | Hou, Jun | eng |
dc.contributor.author | Steinhoff, Christine | eng |
dc.contributor.author | Rijkers, Erikjan | eng |
dc.contributor.author | Lenhard, Boris | eng |
dc.contributor.author | Grosveld, Frank | eng |
dc.date.accessioned | 2014-03-14T11:37:22Z | |
dc.date.available | 2014-03-14T11:37:22Z | |
dc.date.issued | 2010 | eng |
dc.identifier.issn | 0890-9369 | en_US |
dc.identifier.uri | https://hdl.handle.net/1956/7851 | |
dc.description.abstract | One of the complexes formed by the hematopoietic transcription factor Gata1 is a complex with the Ldb1 (LIM domain-binding protein 1) and Tal1 proteins. It is known to be important for the development and differentiation of the erythroid cell lineage and is thought to be implicated in long-range interactions. Here, the dynamics of the composition of the complex—in particular, the binding of the negative regulators Eto2 and Mtgr1—are studied, in the context of their genome-wide targets. This shows that the complex acts almost exclusively as an activator, binding a very specific combination of sequences, with a positioning relative to transcription start site, depending on the type of the core promoter. The activation is accompanied by a net decrease in the relative binding of Eto2 and Mtgr1. A Chromosome Conformation Capture sequencing (3C-seq) assay also shows that the binding of the Ldb1 complex marks genomic interaction sites in vivo. This establishes the Ldb1 complex as a positive regulator of the final steps of erythroid differentiation that acts through the shedding of negative regulators and the active interaction between regulatory sequences. | en_US |
dc.language.iso | eng | eng |
dc.publisher | Cold Spring Harbor Laboratory Press | en_US |
dc.relation.ispartof | <a href="http://hdl.handle.net/1956/7852" target="blank">Analysis of transcription factor complexes and their associated chromatin interactions during erythropoiesis</a> | en_US |
dc.rights | Attribution-NonCommercial CC BY-NC | eng |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/4.0/ | eng |
dc.subject | ChIP sequencing | eng |
dc.subject | transcription factor complexes | eng |
dc.subject | Development | eng |
dc.subject | Differentiation | eng |
dc.subject | erythropoiesis | eng |
dc.subject | long-range interactions | eng |
dc.title | The genome-wide dynamics of the binding of Ldb1 complexes during erythroid differentiation | en_US |
dc.type | Peer reviewed | |
dc.type | Journal article | |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | Copyright 2010 by Cold Spring Harbor Laboratory Press. | en_US |
dc.identifier.doi | https://doi.org/10.1101/gad.551810 | |
dc.identifier.cristin | 348478 | |
dc.source.journal | Genes & Development | |
dc.source.pagenumber | 277-289 | |
dc.source.volume | 24 | |