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dc.contributor.authorStrøm, Gro Elizabeth Annen_US
dc.contributor.authorMoyo, Sabrina Johnen_US
dc.contributor.authorFataki, Maulidien_US
dc.contributor.authorLangeland, Ninaen_US
dc.contributor.authorBlomberg, Bjørnen_US
dc.description.abstractBackground: Monitoring mortality and morbidity attributable to malaria is paramount to achieve elimination of malaria. Diagnosis of malaria is challenging and PCR is a reliable method for identifying malaria with high sensitivity. However, blood specimen collection and transport can be challenging and obtaining dried blood spots (DBS) on filter paper by finger-prick may have advantages over collecting whole blood by venepuncture. Methods: DBS and whole blood were collected from febrile children admitted at the general paediatric wards at a referral hospital in Dar es Salaam, Tanzania. DNA extracted from whole blood and from DBS was tested with a genus-specific PCR targeting the mitochondrial Plasmodium genome. Positive samples by PCR of DNA from whole blood were tested with species-specific PCR targeting the 18S rRNA locus, or sequencing if species-specific PCR was negative. Rapid diagnostic test (RDT) and thin blood smear microscopy was carried out on all patients where remnant whole blood and a blood slide, respectively, were available. Results: Positivity of PCR was 24.5 (78/319) and 11.2% (52/442) by whole blood and DBS, respectively. All samples positive on DBS were also positive on Plasmodium falciparum species-specific PCR. All RDT positive cases were also positive by DBS PCR. All but three cases with positive blood slides were also positive by DBS. Conclusions: In this study, PCR for malaria mitochondrial DNA extracted from whole blood was more sensitive than from DBS. However, DBS are a practical alternative to whole blood and detected approximately the same number of cases as RDTs and, therefore, remain relevant for research purposes.en_US
dc.publisherBioMed Centraleng
dc.relation.ispartof<a href="" target="blank">Diagnosing pediatric malaria in Dar es Salaam, Tanzania. Clinical and laboratorial perspectives</a>eng
dc.rightsAttribution CC BYeng
dc.subjectFilter papereng
dc.subjectDried blood spots/DBSeng
dc.subjectInstaGene Matrixeng
dc.titlePCR targeting Plasmodium mitochondrial genome of DNA extracted from dried blood on filter paper compared to whole blooden_US
dc.typePeer reviewed
dc.typeJournal article
dc.rights.holderCopyright 2014 Strøm et al.; licensee BioMed Central Ltd.
dc.rights.holderGro EA Strøm et al.; licensee BioMed Central Ltd.
dc.source.journalMalaria Journal

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