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dc.contributor.authorMoyo, Sabrina Johnen_US
dc.contributor.authorBlomberg, Bjørnen_US
dc.contributor.authorHanevik, Kurten_US
dc.contributor.authorKommedal, Øyvinden_US
dc.contributor.authorVainio, Kirstien_US
dc.contributor.authorMaselle, Samwel Y.en_US
dc.contributor.authorLangeland, Ninaen_US
dc.description.abstractBackground: Tanzania currently rolls out vaccination against rotavirus-diarrhea, a major cause of child illness and death. As the vaccine covers a limited number of rotavirus variants, this study describes the molecular epidemiology of rotavirus among children under two years in Dar es Salaam, Tanzania, prior to implementation of vaccination. Methods: Stool specimens, demographic and clinical information, were collected from 690 children admitted to hospital due to diarrhea (cases) and 545 children without diarrhea (controls) during one year. Controls were inpatient or children attending child health clinics. Rotavirus antigen was detected using ELISA and positive samples were typed by multiplex semi-nested PCR and sequencing. Results: The prevalence of rotavirus was higher in cases (32.5%) than in controls (7.7%, P,0.001). The most common G genotypes were G1 followed by G8, G12, and G4 in cases and G1, G12 and G8 in controls. The Tanzanian G1 variants displayed 94% similarity with the Rotarix vaccine G1 variant. The commonest P genotypes were P[8], P[4] and P[6], and the commonest G/P combination G1 P[8] (n = 123), G8 P[4] and G12 P[6]. Overall, rotavirus prevalence was higher in cool (23.9%) than hot months (17.1%) of the year (P = 0.012). We also observed significant seasonal variation of G genotypes. Rotavirus was most frequently found in the age group of four to six months. The prevalence of rotavirus in cases was lower in stunted children (28.9%) than in non-stunted children (40.1%, P = 0.003) and lower in HIV-infected (15.4%, 4/26) than in HIVuninfected children (55.3%, 42/76, P,0.001). Conclusion: This pre-vaccination study shows predominance of genotype G1 in Tanzania, which is phylogenetically distantly related to the vaccine strains. We confirm the emergence of genotype G8 and G12. Rotavirus infection and circulating genotypes showed seasonal variation. This study also suggests that rotavirus may not be an opportunistic pathogen in children infected with HIV.en_US
dc.relation.ispartof<a href="" target="blank">Viral diarrhoea in children under two years of age in Dar es Salaam, Tanzania. Clinical and molecular epidemiology</a>eng
dc.rightsAttribution CC BYeng
dc.titleGenetic diversity of circulating rotavirus strains in Tanzania prior to the introduction of vaccinationen_US
dc.typePeer reviewed
dc.typeJournal article
dc.rights.holderCopyright 2014 Moyo et al
dc.source.journalPLoS ONE
dc.subject.nsiVDP::Medical sciences: 700::Health sciences: 800::Preventive medicine: 804eng
dc.subject.nsiVDP::Medical sciences: 700::Clinical medical sciences: 750::Tropical medicine: 761eng
dc.subject.nsiVDP::Medisinske fag: 700::Helsefag: 800::Forebyggende medisin: 804nob
dc.subject.nsiVDP::Medisinske fag: 700::Klinisk medisinske fag: 750::Tropemedisin: 761nob

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