• Activation mechanism and activity of globupain, a thermostable C11 protease from the Arctic Mid-Ocean Ridge hydrothermal system 

      Røyseth, Victoria; M. Hurysz, Brianna; Kaczorowska, Anna-Karina; Dorawa, Sebastian; Fedøy, Anita-Elin; Arsin, Hasan; Sá M. Serafim, Mateus; A. Myers, Samuel; Werbowy, Olesia; Kaczorowski, Tadeusz; Stokke, Runar; O'Donoghue, Anthony J.; Steen, Ida Helene (Journal article; Peer reviewed, 2023)
      Deep-sea hydrothermal vents offer unique habitats for heat tolerant enzymes with potential new enzymatic properties. Here, we present the novel C11 protease globupain, which was prospected from a metagenome-assembled genome ...
    • Bioorthogonal Non-canonical Amino Acid Tagging Combined With Flow Cytometry for Determination of Activity in Aquatic Microorganisms 

      Lindivat, Mathilde; Larsen, Aud; Hess-Erga, Ole-Kristian; Bratbak, Gunnar; Hoell, Ingunn (Journal article; Peer reviewed, 2020)
      In this study, we have combined bioorthogonal non-canonical amino acid tagging (BONCAT) and flow cytometry (FCM) analysis, and we demonstrate the applicability of the method for marine prokaryotes. Enumeration of active ...
    • A closer look into the microbiome of microalgal cultures 

      Steinrücken, Pia; Jackson, Steve; Müller, Oliver; Puntervoll, Pål; Kleinegris, Dorinde Mechtilde Meike (Journal article; Peer reviewed, 2023)
      Although bacteria are commonly co-occurring in microalgal cultivation and production systems, little is known about their community structure and how it might be affected by specific microalgal groups or growth conditions. ...
    • Effects of sample handling and cultivation bias on the specificity of bacterial communities in keratose marine sponges 

      Hardoim, Cristiane; Cardinale, Massimiliano; Cúcio, Ana C. B.; Esteves, Ana I. S.; Berg, Gabriele; Xavier, Joana; Cox, Cymon J.; Costa, Rodrigo (Peer reviewed; Journal article, 2014-11-18)
      Complex and distinct bacterial communities inhabit marine sponges and are believed to be essential to host survival, but our present-day inability to domesticate sponge symbionts in the laboratory hinders our access to the ...
    • The Escherichia coli alkA Gene Is Activated to Alleviate Mutagenesis by an Oxidized Deoxynucleoside 

      Grøsvik, Kristin; Tesfahun, Almaz Nigatu; Muruzábal-Lecumberri, Izaskun; Haugland, Gyri Teien; Leiros, Ingar; Ruoff, Peter; Kvaløy, Jan Terje; Knævelsrud, Ingeborg; Ånensen, Hilde; Alexeeva, Marina; Sato, Kousuke; Matsuda, Akira; Alseth, Ingrun; Klungland, Arne; Bjelland, Svein (Journal article; Peer reviewed, 2020)
      The cellular methyl donor S-adenosylmethionine (SAM) and other endo/exogenous agents methylate DNA bases non-enzymatically into products interfering with replication and transcription. An important product is 3-methyladenine ...
    • Flow Cytometric Analysis of Bacterial Protein Synthesis: Monitoring Vitality After Water Treatment 

      Lindivat, Mathilde; Bratbak, Gunnar; Larsen, Aud; Hess-Erga, Ole-Kristian; Hoell, Ingunn Alne (Journal article; Peer reviewed, 2021)
      Bacterial vitality after water disinfection treatment was investigated using bio-orthogonal non-canonical amino acid tagging (BONCAT) and flow cytometry (FCM). Protein synthesis activity and DNA integrity (BONCAT–SYBR ...
    • Global Biogeographic Distribution Patterns of Thermoacidophilic Verrucomicrobia Methanotrophs Suggest Allopatric Evolution 

      Erikstad, Helge Andre; Ceballos, Ruben Michael; Smestad, Natalie Bennett; Birkeland, Nils-Kåre (Peer reviewed; Journal article, 2019)
      Thermoacidophilic methane-oxidizing Verrucomicrobia of the candidate genus Methylacidiphilum represent a bacterial taxon adapted to highly acidic (pH 1–4) and moderate temperature (∼65∘C) methane-containing geothermal ...
    • Microbial Communities in a Flow-Through Fish Farm for Lumpfish (Cyclopterus lumpus L.) During Healthy Rearing Conditions 

      Roalkvam, Irene; Drønen, Karine; Dahle, Håkon; Wergeland, Heidrun Inger (Peer reviewed; Journal article, 2019-07-12)
      Lumpfish can efficiently remove sea lice from Atlantic salmon in net-pens, and production of lumpfish in closed fish farms is a new, fast developing industry in Norway. However, periodic outbreaks of bacterial diseases in ...
    • Microbial inventory of deeply buried oceanic crust from a young ridge flank 

      Jørgensen, Steffen Leth; Zhao, Rui (Peer reviewed; Journal article, 2016-05-27)
      The deep marine biosphere has over the past decades been exposed as an immense habitat for microorganisms with wide-reaching implications for our understanding of life on Earth. Recent advances in knowledge concerning this ...
    • The Microbiome and Occurrence of Methanotrophy in Carnivorous Sponges 

      Hestetun, Jon Thomassen; Dahle, Håkon; Jørgensen, Steffen Leth; Olsen, Bernt Rydland; Rapp, Hans Tore (Peer reviewed; Journal article, 2016-11-09)
      As shown by recent studies, filter-feeding sponges are known to host a wide variety of microorganisms. However, the microbial community of the non-filtering carnivorous sponges (Porifera, Cladorhizidae) has been the subject ...
    • Phylogeography and host specificity of Pasteurellaceae pathogenic to sea-farmed fish in the north-east Atlantic 

      Gulla, Snorre; Colquhoun, Duncan John; Olsen, Anne Berit; Spilsberg, Bjørn; Lagesen, Karin; Åkesson, Caroline Piercey; Strøm, Sverri; Manji, Farah; Birkbeck, T.H.; Nilsen, Hanne Katrine (Journal article; Peer reviewed, 2023)
      The present study was undertaken to address the recent spate of pasteurellosis outbreaks among sea-farmed Atlantic salmon (Salmo salar) in Norway and Scotland, coinciding with sporadic disease episodes in lumpfish (Cyclopterus ...
    • Population dynamics and characteristics of Klebsiella pneumoniae from healthy poultry in Norway 

      Kaspersen, Håkon; Urdahl, Anne Margrete; Franklin-Alming, Fiona Valerie; Ilag, Hanna Karin; Hetland, Marit; Bernhoff, Eva; Löhr, Iren Høyland; Sunde, Marianne (Journal article; Peer reviewed, 2023-05-18)
      Klebsiella pneumoniae is an important opportunistic pathogen widely studied in relation to human infection and colonization. However, there is a lack of knowledge regarding other niches that K. pneumoniae may inhabit. K. ...
    • Spatiotemporal Dynamics of Ammonia-Oxidizing Thaumarchaeota in Distinct Arctic Water Masses 

      Müller, Oliver; Wilson, Bryan; Paulsen, Maria Lund; Rumińska, Agnieszka; Armo, Hilde R.; Bratbak, Gunnar; Øverås, Lise (Peer reviewed; Journal article, 2018-01-23)
      One of the most abundant archaeal groups on Earth is the Thaumarchaeota. They are recognized as major contributors to marine ammonia oxidation, a crucial step in the biogeochemical cycling of nitrogen. Their universal ...
    • Tailoring Hydrothermal Vent Biodiversity Toward Improved Biodiscovery Using a Novel in situ Enrichment Strategy 

      Stokke, Runar; Reeves, Eoghan; Dahle, Håkon; Fedøy, Anita-Elin; Viflot, Thomas Øfstegaard; Onstad, Solveig L.; Vulcano, Francesca; Pedersen, Rolf Birger; Eijsink, Vincent; Steen, Ida Helene (Journal article; Peer reviewed, 2020)
      Deep-sea hydrothermal vents are amongst the most extreme environments on Earth and represent interesting targets for marine bioprospecting and biodiscovery. The microbial communities in hydrothermal vents are often dominated ...