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dc.contributor.authorKarlsen, Mariuseng
dc.contributor.authorVilloing, Stephaneeng
dc.contributor.authorRimstad, Espeneng
dc.contributor.authorNylund, Areeng
dc.description.abstractSalmonid alphavirus (SAV) causes disease in farmed salmonid fish and is divided into different genetic subtypes (SAV1-6). Here we report the cloning and characterization of the 5'- and 3'- untranslated regions (UTR) of a SAV3 isolated from Atlantic salmon in Norway. The sequences of the UTRs are very similar to those of SAV1 and SAV2, but single nucleotide polymorphisms are present, also in the 3' - conserved sequence element (3'-CSE). Prediction of the RNA secondary structure suggested putative stem-loop structures in both the 5'- and 3'-ends, similar to those of alphaviruses from the terrestrial environment, indicating that the general genome replication initiation strategy for alphaviruses is also utilized by SAV. A DNA replicon vector, pmSAV3, based upon a pVAX1 backbone and the SAV3 genome was constructed, and the SAV3 non-structural proteins were used to express a reporter gene controlled by the SAV3 subgenomic promoter. Transfection of pmSAV3 into CHSE and BF2 cell lines resulted in expression of the reporter protein, confirming that the cloned SAV3 replication apparatus and UTRs are functional in fish cells.en_US
dc.publisherBioMed Centralen_US
dc.rightsAttribution CC BYeng
dc.titleCharacterization of untranslated regions of the salmonid alphavirus 3 (SAV3) genome and construction of a SAV3 based repliconen_US
dc.typePeer reviewed
dc.typeJournal article
dc.description.versionPeer Reviewed
dc.rights.holderMarius Karlsen et al.; licensee BioMed Central Ltd.en_US
dc.rights.holderCopyright 2009 Karlsen et al; licensee BioMed Central Ltden_US
dc.source.journalVirology Journal

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