Now showing items 1-4 of 4

  • Characteristics of 454 pyrosequencing data—enabling realistic simulation with flowsim 

    Balzer, Susanne Mignon; Malde, Ketil; Lanzén, Anders; Sharma, Animesh; Jonassen, Inge (Oxford University Press, 2010)
    Motivation: The commercial launch of 454 pyrosequencing in 2005was a milestone in genome sequencing in terms of performance and cost. Throughout the three available releases, average read lengths have increased to ∼500 ...
  • Filtering duplicate reads from 454 pyrosequencing data 

    Balzer, Susanne Mignon; Malde, Ketil; Grohme, Markus A.; Jonassen, Inge (Oxford University Press, 2013)
    Motivation: Throughout the recent years, 454 pyrosequencing has emerged as an efficient alternative to traditional Sanger sequencing and is widely used in both de novo whole-genome sequencing and metagenomics. Especially ...
  • Repeats and EST analysis for new organisms 

    Malde, Ketil; Jonassen, Inge (BioMed Central, 2008-01-18)
    Background Repeat masking is an important step in the EST analysis pipeline. For new species, genomic knowledge is scarce and good repeat libraries are typically unavailable. In these cases it is common practice to mask ...
  • Systematic exploration of error sources in pyrosequencing flowgram data 

    Balzer, Susanne Mignon; Malde, Ketil; Jonassen, Inge (Oxford University Press, 2011)
    Motivation: 454 pyrosequencing, by Roche Diagnostics, has emerged as an alternative to Sanger sequencing when it comes to read lengths, performance and cost, but shows higher per-base error rates. Although there are ...