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Characterization of hARD2, a processed hARD1 gene duplicate, encoding a human protein N-α-acetyltransferase

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dc.contributor.author Arnesen, Thomas
dc.contributor.author Betts, Matthew J.
dc.contributor.author Pendino, Frédéric
dc.contributor.author Liberles, David A.
dc.contributor.author Anderson, Dave
dc.contributor.author Caro, Jaime
dc.contributor.author Kong, Xianguo
dc.contributor.author Varhaug, Jan Erik
dc.contributor.author Lillehaug, Johan
dc.date.accessioned 2006-10-25T11:26:26Z
dc.date.available 2006-10-25T11:26:26Z
dc.date.issued 2006-04-25
dc.identifier.uri http://dx.doi.org/10.1186/1471-2091-7-13
dc.identifier.uri http://hdl.handle.net/1956/1911
dc.description.abstract Background: Protein acetylation is increasingly recognized as an important mechanism regulating a variety of cellular functions. Several human protein acetyltransferases have been characterized, most of them catalyzing ε-acetylation of histones and transcription factors. We recently described the human protein acetyltransferase hARD1 (human Arrest Defective 1). hARD1 interacts with NATH (N-Acetyl Transferase Human) forming a complex expressing protein N-terminal α-acetylation activity. Results: We here describe a human protein, hARD2, with 81 % sequence identity to hARD1. The gene encoding hARD2 most likely originates from a eutherian mammal specific retrotransposition event. hARD2 mRNA and protein are expressed in several human cell lines. Immunoprecipitation experiments show that hARD2 protein potentially interacts with NATH, suggesting that hARD2-NATH complexes may be responsible for protein N-α-acetylation in human cells. In NB4 cells undergoing retinoic acid mediated differentiation, the level of endogenous hARD1 and NATH protein decreases while the level of hARD2 protein is stable. Conclusion: A human protein N-α-acetyltransferase is herein described. ARD2 potentially complements the functions of ARD1, adding more flexibility and complexity to protein N-α-acetylation in human cells as compared to lower organisms which only have one ARD. en
dc.format.extent 1064704 bytes
dc.format.mimetype application/pdf
dc.language.iso eng en
dc.publisher BioMed Central en
dc.relation.ispartofseries BMC Biochemistry 2006 7(13) en
dc.title Characterization of hARD2, a processed hARD1 gene duplicate, encoding a human protein N-α-acetyltransferase en
dc.type Journal article en
dc.type Peer reviewed en
dc.subject.nsi VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Molekylærbiologi: 473 no


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