BORA - UiB

Bergen Open Research Archive

Characterization of hARD2, a processed hARD1 gene duplicate, encoding a human protein N-α-acetyltransferase

Bergen Open Research Archive

Show simple item record

dc.contributor.author Arnesen, Thomas eng
dc.contributor.author Betts, Matthew J. eng
dc.contributor.author Pendino, Frédéric eng
dc.contributor.author Liberles, David A. eng
dc.contributor.author Anderson, Dave eng
dc.contributor.author Caro, Jaime eng
dc.contributor.author Kong, Xianguo eng
dc.contributor.author Varhaug, Jan Erik eng
dc.contributor.author Lillehaug, Johan eng
dc.date.accessioned 2006-10-25T11:26:26Z
dc.date.available 2006-10-25T11:26:26Z
dc.date.issued 2006-04-25 eng
dc.identifier.issn 1471-2091 eng
dc.identifier.uri http://hdl.handle.net/1956/1911
dc.description.abstract <p>Background: Protein acetylation is increasingly recognized as an important mechanism regulating a variety of cellular functions. Several human protein acetyltransferases have been characterized, most of them catalyzing ε-acetylation of histones and transcription factors. We recently described the human protein acetyltransferase hARD1 (human Arrest Defective 1). hARD1 interacts with NATH (N-Acetyl Transferase Human) forming a complex expressing protein N-terminal α-acetylation activity.</p> <p>Results: We here describe a human protein, hARD2, with 81 % sequence identity to hARD1. The gene encoding hARD2 most likely originates from a eutherian mammal specific retrotransposition event. hARD2 mRNA and protein are expressed in several human cell lines. Immunoprecipitation experiments show that hARD2 protein potentially interacts with NATH, suggesting that hARD2-NATH complexes may be responsible for protein N-α-acetylation in human cells. In NB4 cells undergoing retinoic acid mediated differentiation, the level of endogenous hARD1 and NATH protein decreases while the level of hARD2 protein is stable.</p> <p>Conclusion: A human protein N-α-acetyltransferase is herein described. ARD2 potentially complements the functions of ARD1, adding more flexibility and complexity to protein N-α-acetylation in human cells as compared to lower organisms which only have one ARD.</p> en
dc.format.extent 1064704 bytes eng
dc.format.mimetype application/pdf eng
dc.language.iso eng eng
dc.publisher BioMed Central eng
dc.rights Attribution CC BY eng
dc.rights.uri http://creativecommons.org/licenses/by/2.0 eng
dc.title Characterization of hARD2, a processed hARD1 gene duplicate, encoding a human protein N-α-acetyltransferase eng
dc.type Journal article eng
dc.subject.nsi VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Molekylærbiologi: 473 nob
dc.rights.holder Copyright 2006 Arnesen et al; licensee BioMed Central Ltd.
dc.type.version publishedVersion eng
bora.peerreviewed Peer reviewed eng
bora.journalTitle BMC Biochemistry eng
bibo.volume 7 eng
bora.cristinID 375218 eng
bibo.number 13 eng
bibo.doi http://dx.doi.org/10.1186/1471-2091-7-13 eng
dc.identifier.cristinID 375218 eng
dc.identifier.doi http://dx.doi.org/10.1186/1471-2091-7-13


Files in this item

 
 

This item appears in the following Collection(s)

Show simple item record

Attribution CC BY Except where otherwise noted, this item's license is described as Attribution CC BY

Search BORA


Browse

My Account