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Genomic Insights into Methanotrophy: The Complete Genome Sequence of Methylococcus capsulatus (Bath)

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dc.contributor.author Ward, Naomi eng
dc.contributor.author Larsen, Øivind eng
dc.contributor.author Sakwa, James eng
dc.contributor.author Bruseth, Live eng
dc.contributor.author Khouri, Hoda eng
dc.contributor.author Durkin, A. Scott eng
dc.contributor.author Dimitrov, George eng
dc.contributor.author Jiang, Lingxia eng
dc.contributor.author Scanlan, David eng
dc.contributor.author Kang, Katherine H. eng
dc.contributor.author Lewis, Matt eng
dc.contributor.author Nelson, Karen E. eng
dc.contributor.author Methé, Barbara eng
dc.contributor.author Wu, Martin eng
dc.contributor.author Heidelberg, John F. eng
dc.contributor.author Paulsen, Ian T. eng
dc.contributor.author Fouts, Derrick eng
dc.contributor.author Ravel, Jacques eng
dc.contributor.author Tettelin, Hervé eng
dc.contributor.author Ren, Qinghu eng
dc.contributor.author Read, Tim eng
dc.contributor.author DeBoy, Robert T. eng
dc.contributor.author Seshadri, Rekha eng
dc.contributor.author Salzberg, Steven L. eng
dc.contributor.author Jensen, Harald B. eng
dc.contributor.author Birkeland, Nils-Kåre eng
dc.contributor.author Nelson, William C. eng
dc.contributor.author Dodson, Robert J. eng
dc.contributor.author Grindhaug, Svenn Helge eng
dc.contributor.author Holt, Ingeborg eng
dc.contributor.author Eidhammer, Ingvar eng
dc.contributor.author Jonassen, Inge eng
dc.contributor.author Vanaken, Susan eng
dc.contributor.author Utterback, Terry eng
dc.contributor.author Feldblyum, Tamara V. eng
dc.contributor.author Fraser, Claire M. eng
dc.contributor.author Lillehaug, Johan eng
dc.contributor.author Eisen, Jonathan A. eng
dc.date.accessioned 2006-11-24T17:05:07Z
dc.date.available 2006-11-24T17:05:07Z
dc.date.issued 2004-09-21 eng
dc.identifier.citation PLoS Biology 2(10): e303
dc.identifier.issn 1544-9173 eng
dc.identifier.uri http://hdl.handle.net/1956/1966
dc.description.abstract Methanotrophs are ubiquitous bacteria that can use the greenhouse gas methane as a sole carbon and energy source for growth, thus playing major roles in global carbon cycles, and in particular, substantially reducing emissions of biologically generated methane to the atmosphere. Despite their importance, and in contrast to organisms that play roles in other major parts of the carbon cycle such as photosynthesis, no genome-level studies have been published on the biology of methanotrophs. We report the first complete genome sequence to our knowledge from an obligate methanotroph, Methylococcus capsulatus (Bath), obtained by the shotgun sequencing approach. Analysis revealed a 3.3-Mb genome highly specialized for a methanotrophic lifestyle, including redundant pathways predicted to be involved in methanotrophy and duplicated genes for essential enzymes such as the methane monooxygenases. We used phylogenomic analysis, gene order information, and comparative analysis with the partially sequenced methylotroph Methylobacterium extorquens to detect genes of unknown function likely to be involved in methanotrophy and methylotrophy. Genome analysis suggests the ability of M. capsulatus to scavenge copper (including a previously unreported nonribosomal peptide synthetase) and to use copper in regulation of methanotrophy, but the exact regulatory mechanisms remain unclear. One of the most surprising outcomes of the project is evidence suggesting the existence of previously unsuspected metabolic flexibility in M. capsulatus, including an ability to grow on sugars, oxidize chemolithotrophic hydrogen and sulfur, and live under reduced oxygen tension, all of which have implications for methanotroph ecology. The availability of the complete genome of M. capsulatus (Bath) deepens our understanding of methanotroph biology and its relationship to global carbon cycles. We have gained evidence for greater metabolic flexibility than was previously known, and for genetic components that may have biotechnological potential. en
dc.format.extent 362419 bytes eng
dc.format.mimetype application/pdf eng
dc.language.iso eng eng
dc.publisher Public Library of Science eng
dc.title Genomic Insights into Methanotrophy: The Complete Genome Sequence of Methylococcus capsulatus (Bath) eng
dc.type Journal article eng
dc.type Peer reviewed eng
dc.subject.nsi VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470 nob
bora.peerreviewed Peer reviewed eng
bibo.doi http://dx.doi.org/10.1371/journal.pbio.0020303 eng
dc.identifier.doi http://dx.doi.org/10.1371/journal.pbio.0020303


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