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dc.contributor.authorWard, Naomieng
dc.contributor.authorLarsen, Øivindeng
dc.contributor.authorSakwa, Jameseng
dc.contributor.authorBruseth, Liveeng
dc.contributor.authorKhouri, Hodaeng
dc.contributor.authorDurkin, A. Scotteng
dc.contributor.authorDimitrov, Georgeeng
dc.contributor.authorJiang, Lingxiaeng
dc.contributor.authorScanlan, Davideng
dc.contributor.authorKang, Katherine H.eng
dc.contributor.authorLewis, Matteng
dc.contributor.authorNelson, Karen E.eng
dc.contributor.authorMethé, Barbaraeng
dc.contributor.authorWu, Martineng
dc.contributor.authorHeidelberg, John F.eng
dc.contributor.authorPaulsen, Ian T.eng
dc.contributor.authorFouts, Derrickeng
dc.contributor.authorRavel, Jacqueseng
dc.contributor.authorTettelin, Hervéeng
dc.contributor.authorRen, Qinghueng
dc.contributor.authorRead, Timeng
dc.contributor.authorDeBoy, Robert T.eng
dc.contributor.authorSeshadri, Rekhaeng
dc.contributor.authorSalzberg, Steven L.eng
dc.contributor.authorJensen, Harald B.eng
dc.contributor.authorBirkeland, Nils-Kåreeng
dc.contributor.authorNelson, William C.eng
dc.contributor.authorDodson, Robert J.eng
dc.contributor.authorGrindhaug, Svenn Helgeeng
dc.contributor.authorHolt, Ingeborgeng
dc.contributor.authorEidhammer, Ingvareng
dc.contributor.authorJonassen, Ingeeng
dc.contributor.authorVanaken, Susaneng
dc.contributor.authorUtterback, Terryeng
dc.contributor.authorFeldblyum, Tamara V.eng
dc.contributor.authorFraser, Claire M.eng
dc.contributor.authorLillehaug, Johaneng
dc.contributor.authorEisen, Jonathan A.eng
dc.date.accessioned2006-11-24T17:05:07Z
dc.date.available2006-11-24T17:05:07Z
dc.date.issued2004-09-21eng
dc.identifier.citationPLoS Biology 2(10): e303
dc.identifier.issn1544-9173eng
dc.identifier.urihttp://hdl.handle.net/1956/1966
dc.description.abstractMethanotrophs are ubiquitous bacteria that can use the greenhouse gas methane as a sole carbon and energy source for growth, thus playing major roles in global carbon cycles, and in particular, substantially reducing emissions of biologically generated methane to the atmosphere. Despite their importance, and in contrast to organisms that play roles in other major parts of the carbon cycle such as photosynthesis, no genome-level studies have been published on the biology of methanotrophs. We report the first complete genome sequence to our knowledge from an obligate methanotroph, Methylococcus capsulatus (Bath), obtained by the shotgun sequencing approach. Analysis revealed a 3.3-Mb genome highly specialized for a methanotrophic lifestyle, including redundant pathways predicted to be involved in methanotrophy and duplicated genes for essential enzymes such as the methane monooxygenases. We used phylogenomic analysis, gene order information, and comparative analysis with the partially sequenced methylotroph Methylobacterium extorquens to detect genes of unknown function likely to be involved in methanotrophy and methylotrophy. Genome analysis suggests the ability of M. capsulatus to scavenge copper (including a previously unreported nonribosomal peptide synthetase) and to use copper in regulation of methanotrophy, but the exact regulatory mechanisms remain unclear. One of the most surprising outcomes of the project is evidence suggesting the existence of previously unsuspected metabolic flexibility in M. capsulatus, including an ability to grow on sugars, oxidize chemolithotrophic hydrogen and sulfur, and live under reduced oxygen tension, all of which have implications for methanotroph ecology. The availability of the complete genome of M. capsulatus (Bath) deepens our understanding of methanotroph biology and its relationship to global carbon cycles. We have gained evidence for greater metabolic flexibility than was previously known, and for genetic components that may have biotechnological potential.en
dc.format.extent362419 byteseng
dc.format.mimetypeapplication/pdfeng
dc.language.isoengeng
dc.publisherPublic Library of Scienceeng
dc.titleGenomic Insights into Methanotrophy: The Complete Genome Sequence of Methylococcus capsulatus (Bath)eng
dc.typeJournal articleeng
dc.typePeer reviewedeng
dc.subject.nsiVDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470nob
bora.peerreviewedPeer reviewedeng
bibo.doihttp://dx.doi.org/10.1371/journal.pbio.0020303eng
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pbio.0020303


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