Bergen Open Research Archive

Genomic Insights into Methanotrophy: The Complete Genome Sequence of Methylococcus capsulatus (Bath)

Bergen Open Research Archive

Show simple item record Ward, Naomi Larsen, Øivind Sakwa, James Bruseth, Live Khouri, Hoda Durkin, A. Scott Dimitrov, George Jiang, Lingxia Scanlan, David Kang, Katherine H. Lewis, Matt Nelson, Karen E. Methé, Barbara Wu, Martin Heidelberg, John F. Paulsen, Ian T. Fouts, Derrick Ravel, Jacques Tettelin, Hervé Ren, Qinghu Read, Tim DeBoy, Robert T. Seshadri, Rekha Salzberg, Steven L. Jensen, Harald B. Birkeland, Nils-Kåre Nelson, William C. Dodson, Robert J. Grindhaug, Svenn Helge Holt, Ingeborg Eidhammer, Ingvar Jonassen, Inge Vanaken, Susan Utterback, Terry Feldblyum, Tamara V. Fraser, Claire M. Lillehaug, Johan Eisen, Jonathan A. 2006-11-24T17:05:07Z 2006-11-24T17:05:07Z 2004-09-21
dc.identifier.citation PLoS Biology 2(10): e303
dc.identifier.issn 1544-9173
dc.description.abstract Methanotrophs are ubiquitous bacteria that can use the greenhouse gas methane as a sole carbon and energy source for growth, thus playing major roles in global carbon cycles, and in particular, substantially reducing emissions of biologically generated methane to the atmosphere. Despite their importance, and in contrast to organisms that play roles in other major parts of the carbon cycle such as photosynthesis, no genome-level studies have been published on the biology of methanotrophs. We report the first complete genome sequence to our knowledge from an obligate methanotroph, Methylococcus capsulatus (Bath), obtained by the shotgun sequencing approach. Analysis revealed a 3.3-Mb genome highly specialized for a methanotrophic lifestyle, including redundant pathways predicted to be involved in methanotrophy and duplicated genes for essential enzymes such as the methane monooxygenases. We used phylogenomic analysis, gene order information, and comparative analysis with the partially sequenced methylotroph Methylobacterium extorquens to detect genes of unknown function likely to be involved in methanotrophy and methylotrophy. Genome analysis suggests the ability of M. capsulatus to scavenge copper (including a previously unreported nonribosomal peptide synthetase) and to use copper in regulation of methanotrophy, but the exact regulatory mechanisms remain unclear. One of the most surprising outcomes of the project is evidence suggesting the existence of previously unsuspected metabolic flexibility in M. capsulatus, including an ability to grow on sugars, oxidize chemolithotrophic hydrogen and sulfur, and live under reduced oxygen tension, all of which have implications for methanotroph ecology. The availability of the complete genome of M. capsulatus (Bath) deepens our understanding of methanotroph biology and its relationship to global carbon cycles. We have gained evidence for greater metabolic flexibility than was previously known, and for genetic components that may have biotechnological potential. en
dc.format.extent 362419 bytes
dc.format.mimetype application/pdf
dc.language.iso eng en
dc.publisher Public Library of Science en
dc.title Genomic Insights into Methanotrophy: The Complete Genome Sequence of Methylococcus capsulatus (Bath) en
dc.type Journal article en
dc.type Peer reviewed en
dc.subject.nsi VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470 no

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