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A Mouse Model for Slowly Progressive Primary Tuberculosis

Bergen Open Research Archive

Show simple item record Mustafa, Tehmina Phyu, S. Nilsen, Rune Jonsson, Roland Bjune, Gunnar Aksel 2007-03-09T12:54:19Z 2007-03-09T12:54:19Z 1999-08
dc.identifier.citation Scandinavian Journal of Immunology 50(2):127–136
dc.identifier.issn 0300-9475
dc.identifier.issn 1365-3083
dc.description.abstract The progression from primary Mycobacterium tuberculosis infection to disease is usually slow in humans. The aim of this study was to develop and characterize a mouse model for slowly progressive primary tuberculosis, using the intraperitoneal (i.p.) route of infection, and to compare it with our previously described model of latent M. tuberculosis infection. B6D2F1 hybrid mice inoculated with 1.5 × 106 colony-forming units (CFUs) of M. tuberculosis H37Rv were followed-up for 70 weeks. Lungs, livers and spleens were examined for bacillary growth, histopathological changes and mycobacterial antigens (MPT64, ManLAM and multiple antigens of M. tuberculosis), by immunohistochemical staining. The infection was found to pass through three distinctive phases. During phase 1, mice were healthy despite development of small granulomas and an increasing number of bacilli in the lungs. During phase 2, mice were unwell but mortality was low. The count of M. tuberculosis and the granuloma size stabilized. The granulomas contained an increasing population of large, vacuolated macrophages. During phase 3, mice became moribund and died rapidly, but the M. tuberculosis count remained relatively stable. The inflammatory infiltrates filled ≈ 80% of the lung parenchyma and the lesions were not well demarcated. Rapidly progressing inflammation, rather than an increase in the M. tuberculosis count, seems to contribute more to mortality. en
dc.format.extent 724923 bytes
dc.format.mimetype application/pdf
dc.language.iso eng en
dc.publisher Blackwell Publishing, Inc. en
dc.title A Mouse Model for Slowly Progressive Primary Tuberculosis en
dc.type Journal article en
dc.type Peer reviewed
dc.subject.nsi VDP::Medisinske Fag: 700::Helsefag: 800 en

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