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Switch from stress response to homeobox transcription factors in Adipose tissue after profound fat loss

Bergen Open Research Archive

Show simple item record Dankel, Simon N. eng Fadnes, Dag J. eng Stavrum, Anne-Kristin eng Stansberg, Christine eng Holdhus, Rita eng Hoang, Tuyen Thi Van eng Veum, Vivian L. eng Christensen, Bjørn Jostein eng Våge, Villy eng Sagen, Jørn V. eng Steen, Vidar Martin eng Mellgren, Gunnar eng 2010-12-14T09:18:54Z 2010-12-14T09:18:54Z 2010-06-09
dc.identifier.citation PLoS ONE 5(6): e11033 en
dc.identifier.issn 1932-6203
dc.description.abstract Background In obesity, impaired adipose tissue function may promote secondary disease through ectopic lipid accumulation and excess release of adipokines, resulting in systemic low-grade inflammation, insulin resistance and organ dysfunction. However, several of the genes regulating adipose tissue function in obesity are yet to be identified. Methodology/Principal Findings In order to identify novel candidate genes that may regulate adipose tissue function, we analyzed global gene expression in abdominal subcutaneous adipose tissue before and one year after bariatric surgery (biliopancreatic diversion with duodenal switch, BPD/DS) (n = 16). Adipose tissue from lean healthy individuals was also analyzed (n = 13). Two different microarray platforms (AB 1700 and Illumina) were used to measure the differential gene expression, and the results were further validated by qPCR. Surgery reduced BMI from 53.3 to 33.1 kg/m2. The majority of differentially expressed genes were down-regulated after profound fat loss, including transcription factors involved in stress response, inflammation, and immune cell function (e.g., FOS, JUN, ETS, C/EBPB, C/EBPD). Interestingly, a distinct set of genes was up-regulated after fat loss, including homeobox transcription factors (IRX3, IRX5, HOXA5, HOXA9, HOXB5, HOXC6, EMX2, PRRX1) and extracellular matrix structural proteins (COL1A1, COL1A2, COL3A1, COL5A1, COL6A3). Conclusions/Significance The data demonstrate a marked switch of transcription factors in adipose tissue after profound fat loss, providing new molecular insight into a dichotomy between stress response and metabolically favorable tissue development. Our findings implicate homeobox transcription factors as important regulators of adipose tissue function. en
dc.language.iso eng eng
dc.publisher Public Library of Science en
dc.rights Copyright 2010 Dankel et al. en
dc.rights.uri eng
dc.title Switch from stress response to homeobox transcription factors in Adipose tissue after profound fat loss en
dc.type Peer reviewed eng
dc.type Journal article eng
dc.subject.nsi VDP::Medisinske Fag: 700::Klinisk medisinske fag: 750 nob
dc.subject.nsi VDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Medisinsk genetikk: 714 nob
dc.rightsHolder Dankel et al.
dc.type.version publishedVersion eng
bora.peerreviewed Peer reviewed eng
bora.cristinID 338864

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