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Switch from stress response to homeobox transcription factors in Adipose tissue after profound fat loss

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dc.contributor.author Dankel, Simon N.
dc.contributor.author Fadnes, Dag J.
dc.contributor.author Stavrum, Anne-Kristin
dc.contributor.author Stansberg, Christine
dc.contributor.author Holdhus, Rita
dc.contributor.author Hoang, Tuyen Thi Van
dc.contributor.author Veum, Vivian L.
dc.contributor.author Christensen, Bjørn Jostein
dc.contributor.author Våge, Villy
dc.contributor.author Sagen, Jørn V.
dc.contributor.author Steen, Vidar Martin
dc.contributor.author Mellgren, Gunnar
dc.date.accessioned 2010-12-14T09:18:54Z
dc.date.available 2010-12-14T09:18:54Z
dc.date.issued 2010-06-09
dc.identifier.citation PLoS ONE 5(6): e11033 en
dc.identifier.issn 1932-6203
dc.identifier.uri http://dx.doi.org/10.1371/journal.pone.0011033
dc.identifier.uri http://hdl.handle.net/1956/4346
dc.description.abstract Background In obesity, impaired adipose tissue function may promote secondary disease through ectopic lipid accumulation and excess release of adipokines, resulting in systemic low-grade inflammation, insulin resistance and organ dysfunction. However, several of the genes regulating adipose tissue function in obesity are yet to be identified. Methodology/Principal Findings In order to identify novel candidate genes that may regulate adipose tissue function, we analyzed global gene expression in abdominal subcutaneous adipose tissue before and one year after bariatric surgery (biliopancreatic diversion with duodenal switch, BPD/DS) (n = 16). Adipose tissue from lean healthy individuals was also analyzed (n = 13). Two different microarray platforms (AB 1700 and Illumina) were used to measure the differential gene expression, and the results were further validated by qPCR. Surgery reduced BMI from 53.3 to 33.1 kg/m2. The majority of differentially expressed genes were down-regulated after profound fat loss, including transcription factors involved in stress response, inflammation, and immune cell function (e.g., FOS, JUN, ETS, C/EBPB, C/EBPD). Interestingly, a distinct set of genes was up-regulated after fat loss, including homeobox transcription factors (IRX3, IRX5, HOXA5, HOXA9, HOXB5, HOXC6, EMX2, PRRX1) and extracellular matrix structural proteins (COL1A1, COL1A2, COL3A1, COL5A1, COL6A3). Conclusions/Significance The data demonstrate a marked switch of transcription factors in adipose tissue after profound fat loss, providing new molecular insight into a dichotomy between stress response and metabolically favorable tissue development. Our findings implicate homeobox transcription factors as important regulators of adipose tissue function. en
dc.language.iso eng en
dc.publisher Public Library of Science en
dc.rights Copyright 2010 Dankel et al. en
dc.rights.uri http://creativecommons.org/licenses/by/2.5/ en
dc.title Switch from stress response to homeobox transcription factors in Adipose tissue after profound fat loss en
dc.type Peer reviewed en
dc.type Journal article en
dc.subject.nsi VDP::Medisinske Fag: 700::Klinisk medisinske fag: 750 en
dc.subject.nsi VDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Medisinsk genetikk: 714 en
dc.rightsHolder Dankel et al.
dc.type.version publishedVersion en
bora.cristinID 338864


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