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Roles of Myosin Va and Rab3D in Membrane Remodeling of Immature Secretory Granules

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dc.contributor.author Kögel, Tanja
dc.contributor.author Gerdes, Hans-Hermann
dc.date.accessioned 2011-03-11T10:53:53Z
dc.date.available 2011-03-11T10:53:53Z
dc.date.issued 2010-12-22
dc.identifier.citation Cellular and Molecular Neurobiology 30: 1303-1308 en_US
dc.identifier.issn 0272-4340
dc.identifier.uri http://dx.doi.org/10.1007/s10571-010-9597-6
dc.identifier.uri http://hdl.handle.net/1956/4571
dc.description.abstract Neuroendocrine secretory granules (SGs) are formed at the trans-Golgi network (TGN) as immature intermediates. In PC12 cells, these immature SGs (ISGs) are transported within seconds to the cell cortex, where they move along actin filaments and complete maturation. This maturation process comprises acidification-dependent processing of cargo proteins, condensation of the SG matrix, and removal of membrane and proteins not destined to mature SGs (MSGs) into ISG-derived vesicles (IDVs). We investigated the roles of myosin Va and Rab3 isoforms in the maturation of ISGs in neuroendocrine PC12 cells. The expression of dominant-negative mutants of myosin Va or Rab3D blocked the removal of the endoprotease furin from ISGs. Furthermore, expression of mutant Rab3D, but not of mutant myosin Va, impaired cargo processing of SGs. In conclusion, our data suggest an implication of myosin Va and Rab3D in the maturation of SGs where they participate in overlapping but not identical tasks. en_US
dc.language.iso eng en_US
dc.publisher Springer en
dc.rights Copyright The Author(s) 2010. This article is published with open access at Springerlink.com en_US
dc.rights.uri http://creativecommons.org/licenses/by-nc/2.5/ en_US
dc.subject Dense core vesicle en
dc.subject Endocrine - Exocytosis en
dc.subject Furin en
dc.subject Hormone en
dc.subject Maturation en
dc.subject Myosin Va en
dc.subject Noc2 en
dc.subject Processing en
dc.subject Rab3 en
dc.subject Rab27 en
dc.subject Secretory granule en
dc.title Roles of Myosin Va and Rab3D in Membrane Remodeling of Immature Secretory Granules en_US
dc.type Peer reviewed en_US
dc.type Journal article en_US
dc.subject.nsi VDP::Medical disciplines: 700 en_US
dc.rightsHolder The Author(s) 2010 en_US
dc.type.version publishedVersion en_US
bora.cristinID 835206


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Copyright The Author(s) 2010. This article is published with open access at Springerlink.com Except where otherwise noted, this item's license is described as Copyright The Author(s) 2010. This article is published with open access at Springerlink.com

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