Show simple item record

dc.contributor.authorKögel, Tanjaeng
dc.contributor.authorGerdes, Hans-Hermanneng
dc.date.accessioned2011-03-11T10:53:53Z
dc.date.available2011-03-11T10:53:53Z
dc.date.issued2010-12-22eng
dc.identifier.citationCellular and Molecular Neurobiology 30: 1303-1308en_US
dc.identifier.issn0272-4340eng
dc.identifier.urihttp://hdl.handle.net/1956/4571
dc.description.abstractNeuroendocrine secretory granules (SGs) are formed at the trans-Golgi network (TGN) as immature intermediates. In PC12 cells, these immature SGs (ISGs) are transported within seconds to the cell cortex, where they move along actin filaments and complete maturation. This maturation process comprises acidification-dependent processing of cargo proteins, condensation of the SG matrix, and removal of membrane and proteins not destined to mature SGs (MSGs) into ISG-derived vesicles (IDVs). We investigated the roles of myosin Va and Rab3 isoforms in the maturation of ISGs in neuroendocrine PC12 cells. The expression of dominant-negative mutants of myosin Va or Rab3D blocked the removal of the endoprotease furin from ISGs. Furthermore, expression of mutant Rab3D, but not of mutant myosin Va, impaired cargo processing of SGs. In conclusion, our data suggest an implication of myosin Va and Rab3D in the maturation of SGs where they participate in overlapping but not identical tasks.en_US
dc.language.isoengeng
dc.publisherSpringereng
dc.rightsAttribution-NonCommercial CC BY-NCeng
dc.rights.urihttp://creativecommons.org/licenses/by-nc/2.5/eng
dc.subjectDense core vesicleeng
dc.subjectEndocrine - Exocytosiseng
dc.subjectFurineng
dc.subjectHormoneeng
dc.subjectMaturationeng
dc.subjectMyosin Vaeng
dc.subjectNoc2eng
dc.subjectProcessingeng
dc.subjectRab3eng
dc.subjectRab27eng
dc.subjectSecretory granuleeng
dc.titleRoles of Myosin Va and Rab3D in Membrane Remodeling of Immature Secretory Granuleseng
dc.typePeer reviewedeng
dc.typeJournal articleeng
dc.subject.nsiVDP::Medical disciplines: 700eng
dc.rights.holderCopyright The Author(s) 2010. This article is published with open access at Springerlink.com
dc.rights.holderThe Author(s) 2010eng
dc.type.versionpublishedVersioneng
bora.peerreviewedPeer reviewedeng
bora.cristinID835206eng
bibo.doihttp://dx.doi.org/10.1007/s10571-010-9597-6eng
dc.identifier.cristinID835206eng
dc.identifier.doihttp://dx.doi.org/10.1007/s10571-010-9597-6


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial CC BY-NC
Except where otherwise noted, this item's license is described as Attribution-NonCommercial CC BY-NC