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Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv

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dc.contributor.author Målen, Hiwa eng
dc.contributor.author Pathak, Sharad eng
dc.contributor.author Søfteland, Tina eng
dc.contributor.author De Souza, Gustavo A. eng
dc.contributor.author Wiker, Harald G. eng
dc.date.accessioned 2011-04-26T07:48:08Z
dc.date.available 2011-04-26T07:48:08Z
dc.date.issued 2010-04-29 eng
dc.identifier.citation BMC Microbiology 10:132 en_US
dc.identifier.issn 1471-2180 eng
dc.identifier.uri http://hdl.handle.net/1956/4690
dc.description.abstract Background Membrane- and membrane-associated proteins are important for the pathogenicity of bacteria. We have analysed the content of these proteins in virulent Mycobacterium tuberculosis H37Rv using Triton X-114 detergent-phase separation for extraction of lipophilic proteins, followed by their identification with high resolution mass spectrometry. Results In total, 1417 different proteins were identified. In silico analysis of the identified proteins revealed that 248 proteins had at least one predicted trans-membrane region. Also, 64 of the identified proteins were predicted lipoproteins, and 54 proteins were predicted as outer membrane proteins. Three-hundred-and-ninety-five of the observed proteins, including 91 integral membrane proteins were described for the first time. Comparison of abundance levels of the identified proteins was performed using the exponentially modified protein abundance index (emPAI) which takes into account the number of the observable peptides to the number of experimentally observed peptide ions for a given protein. The outcome showed that among the membrane-and membrane-associated proteins several proteins are present with high relative abundance. Further, a close examination of the lipoprotein LpqG (Rv3623) which is only detected in the membrane fractions of M. tuberculosis but not in M. bovis, revealed that the homologous gene in M. bovis lack the signal peptide and lipobox motif, suggesting impaired export to the membrane. Conclusions Altogether, we have identified a substantial proportion of membrane- and membrane-associated proteins of M. tuberculosis H37Rv, compared the relative abundance of the identified proteins and also revealed subtle differences between the different members of the M. tuberculosis complex. en_US
dc.language.iso eng eng
dc.publisher BioMed Central eng
dc.rights Attribution CC BY eng
dc.rights.uri http://creativecommons.org/licenses/by/2.0 eng
dc.title Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv eng
dc.type Journal article eng
dc.type Peer reviewed eng
dc.rights.holder Copyright 2010 Målen et al; licensee BioMed Central Ltd.
dc.rights.holder Målen et al. eng
dc.type.version publishedVersion eng
bora.peerreviewed Peer reviewed eng
bora.cristinID 407309 eng
bibo.doi http://dx.doi.org/10.1186/1471-2180-10-132 eng
dc.identifier.cristinID 407309 eng
dc.identifier.doi http://dx.doi.org/10.1186/1471-2180-10-132


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