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Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv

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dc.contributor.author Målen, Hiwa
dc.contributor.author Pathak, Sharad
dc.contributor.author Søfteland, Tina
dc.contributor.author De Souza, Gustavo A.
dc.contributor.author Wiker, Harald G.
dc.date.accessioned 2011-04-26T07:48:08Z
dc.date.available 2011-04-26T07:48:08Z
dc.date.issued 2010-04-29
dc.identifier.citation BMC Microbiology 10:132 en_US
dc.identifier.issn 1471-2180
dc.identifier.uri http://dx.doi.org/10.1186/1471-2180-10-132
dc.identifier.uri http://hdl.handle.net/1956/4690
dc.description.abstract Background Membrane- and membrane-associated proteins are important for the pathogenicity of bacteria. We have analysed the content of these proteins in virulent Mycobacterium tuberculosis H37Rv using Triton X-114 detergent-phase separation for extraction of lipophilic proteins, followed by their identification with high resolution mass spectrometry. Results In total, 1417 different proteins were identified. In silico analysis of the identified proteins revealed that 248 proteins had at least one predicted trans-membrane region. Also, 64 of the identified proteins were predicted lipoproteins, and 54 proteins were predicted as outer membrane proteins. Three-hundred-and-ninety-five of the observed proteins, including 91 integral membrane proteins were described for the first time. Comparison of abundance levels of the identified proteins was performed using the exponentially modified protein abundance index (emPAI) which takes into account the number of the observable peptides to the number of experimentally observed peptide ions for a given protein. The outcome showed that among the membrane-and membrane-associated proteins several proteins are present with high relative abundance. Further, a close examination of the lipoprotein LpqG (Rv3623) which is only detected in the membrane fractions of M. tuberculosis but not in M. bovis, revealed that the homologous gene in M. bovis lack the signal peptide and lipobox motif, suggesting impaired export to the membrane. Conclusions Altogether, we have identified a substantial proportion of membrane- and membrane-associated proteins of M. tuberculosis H37Rv, compared the relative abundance of the identified proteins and also revealed subtle differences between the different members of the M. tuberculosis complex. en_US
dc.language.iso eng en_US
dc.publisher BioMed Central en
dc.rights Copyright 2010 Målen et al; licensee BioMed Central Ltd. en_US
dc.rights.uri http://creativecommons.org/licenses/by/2.0 en_US
dc.title Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv en_US
dc.type Journal article en_US
dc.type Peer reviewed en_US
dc.rightsHolder Målen et al. en_US
dc.type.version publishedVersion en_US
bora.cristinID 407309


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Copyright 2010 Målen et al; licensee BioMed Central Ltd. Except where otherwise noted, this item's license is described as Copyright 2010 Målen et al; licensee BioMed Central Ltd.

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