Show simple item record

dc.contributor.authorMålen, Hiwaeng
dc.contributor.authorPathak, Sharadeng
dc.contributor.authorSøfteland, Tinaeng
dc.contributor.authorDe Souza, Gustavo A.eng
dc.contributor.authorWiker, Harald G.eng
dc.identifier.citationBMC Microbiology 10:132en_US
dc.description.abstractBackground Membrane- and membrane-associated proteins are important for the pathogenicity of bacteria. We have analysed the content of these proteins in virulent Mycobacterium tuberculosis H37Rv using Triton X-114 detergent-phase separation for extraction of lipophilic proteins, followed by their identification with high resolution mass spectrometry. Results In total, 1417 different proteins were identified. In silico analysis of the identified proteins revealed that 248 proteins had at least one predicted trans-membrane region. Also, 64 of the identified proteins were predicted lipoproteins, and 54 proteins were predicted as outer membrane proteins. Three-hundred-and-ninety-five of the observed proteins, including 91 integral membrane proteins were described for the first time. Comparison of abundance levels of the identified proteins was performed using the exponentially modified protein abundance index (emPAI) which takes into account the number of the observable peptides to the number of experimentally observed peptide ions for a given protein. The outcome showed that among the membrane-and membrane-associated proteins several proteins are present with high relative abundance. Further, a close examination of the lipoprotein LpqG (Rv3623) which is only detected in the membrane fractions of M. tuberculosis but not in M. bovis, revealed that the homologous gene in M. bovis lack the signal peptide and lipobox motif, suggesting impaired export to the membrane. Conclusions Altogether, we have identified a substantial proportion of membrane- and membrane-associated proteins of M. tuberculosis H37Rv, compared the relative abundance of the identified proteins and also revealed subtle differences between the different members of the M. tuberculosis complex.en_US
dc.publisherBioMed Centraleng
dc.rightsAttribution CC BYeng
dc.titleDefinition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rveng
dc.typeJournal articleeng
dc.typePeer reviewedeng
dc.rights.holderCopyright 2010 Målen et al; licensee BioMed Central Ltd.
dc.rights.holderMålen et al.eng
bora.peerreviewedPeer reviewedeng

Files in this item


This item appears in the following Collection(s)

Show simple item record

Attribution CC BY
Except where otherwise noted, this item's license is described as Attribution CC BY