Vis enkel innførsel

dc.contributor.authorMoosmann, Alexandraeng
dc.contributor.authorCampsteijn, Coeneng
dc.contributor.authorJansen, Pascal W. T. C.eng
dc.contributor.authorNasrallah, Caroleeng
dc.contributor.authorRaasholm, Martinaeng
dc.contributor.authorStunnenberg, Henk Geng
dc.contributor.authorThompson, Eric M.eng
dc.date.accessioned2012-01-23T14:15:39Z
dc.date.available2012-01-23T14:15:39Z
dc.date.issued2011-07-15eng
dc.PublishedBMC Evolutionary Biology 2011, 11:208en
dc.identifier.issn1471-2148en_US
dc.identifier.urihttp://hdl.handle.net/1956/5507
dc.description.abstractBackground: Histone variants alter the composition of nucleosomes and play crucial roles in transcription, chromosome segregation, DNA repair, and sperm compaction. Modification of metazoan histone variant lineages occurs on a background of genome architecture that shows global similarities from sponges to vertebrates, but the urochordate, Oikopleura dioica, a member of the sister group to vertebrates, exhibits profound modification of this ancestral architecture. Results: We show that a histone complement of 47 gene loci encodes 31 histone variants, grouped in distinct sets of developmental expression profiles throughout the life cycle. A particularly diverse array of 15 male-specific histone variants was uncovered, including a testes-specific H4t, the first metazoan H4 sequence variant reported. Universal histone variants H3.3, CenH3, and H2A.Z are present but O. dioica lacks homologs of macroH2A and H2AX. The genome encodes many H2A and H2B variants and the repertoire of H2A.Z isoforms is expanded through alternative splicing, incrementally regulating the number of acetylatable lysine residues in the functionally important N-terminal “charge patch”. Mass spectrometry identified 40 acetylation, methylation and ubiquitylation posttranslational modifications (PTMs) and showed that hallmark PTMs of “active” and “repressive” chromatin were present in O. dioica. No obvious reduction in silent heterochromatic marks was observed despite high gene density in this extraordinarily compacted chordate genome. Conclusions: These results show that histone gene complements and their organization differ considerably even over modest phylogenetic distances. Substantial innovation among all core and linker histone variants has evolved in concert with adaptation of specific life history traits in this rapidly evolving chordate lineage. Keywords: histone complement, DNA repair, urochordate, posttranslational modification, endocycle, gametogenesis, testes, H2A.Z, alternative splicingen_US
dc.language.isoengeng
dc.publisherBioMed Centralen_US
dc.rightsAttribution CC BYeng
dc.rights.urihttp://creativecommons.org/licenses/by/2.0/eng
dc.titleHistone variant innovation in a rapidly evolving chordate lineageen_US
dc.typePeer reviewed
dc.typeJournal article
dc.description.versionpublishedVersionen_US
dc.rights.holderCopyright 2011 Moosmann et al; licensee BioMed Central Ltd.en_US
dc.identifier.doihttps://doi.org/10.1186/1471-2148-11-208
dc.identifier.cristin850704
dc.subject.nsiVDP::Mathematics and natural science: 400::Basic biosciences: 470::Genetics and genomics: 474en_US


Tilhørende fil(er)

Thumbnail
Thumbnail
Thumbnail

Denne innførselen finnes i følgende samling(er)

Vis enkel innførsel

Attribution CC BY
Med mindre annet er angitt, så er denne innførselen lisensiert som Attribution CC BY