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dc.contributor.authorHunt, James H.en_US
dc.contributor.authorMutti, Navdeep S.en_US
dc.contributor.authorHavukainen, Helien_US
dc.contributor.authorHenshaw, Michael T.en_US
dc.contributor.authorAmdam, Gro V.en_US
dc.date.accessioned2012-02-27T10:21:15Z
dc.date.available2012-02-27T10:21:15Z
dc.date.issued2011-11-01eng
dc.PublishedPLoS ONE 6(11): e26641en
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/1956/5642
dc.description.abstractRecent advancements in genomics provide new tools for evolutionary ecological research. The paper wasp genus Polistes is a model for social insect evolution and behavioral ecology. We developed RNA interference (RNAi)-mediated gene silencing to explore proposed connections between expression of hexameric storage proteins and worker vs. gyne (potential future foundress) castes in naturally-founded colonies of P. metricus. We extended four fragments of putative hexamerin-encoding P. metricus transcripts acquired from a previous study and fully sequenced a gene that encodes Hexamerin 2, one of two proposed hexameric storage proteins of P. metricus. MALDI-TOF/TOF, LC-MSMS, deglycosylation, and detection of phosphorylation assays showed that the two putative hexamerins diverge in peptide sequence and biochemistry. We targeted the hexamerin 2 gene in 5th (last)-instar larvae by feeding RNAi-inducing double-stranded hexamerin 2 RNA directly to larvae in naturally-founded colonies in the field. Larval development and adult traits were not significantly altered in hexamerin 2 knockdowns, but there were suggestive trends toward increased developmental time and less developed ovaries, which are gyne characteristics. By demonstrating how data acquisition from 454/Roche pyrosequencing can be combined with biochemical and proteomics assays and how RNAi can be deployed successfully in field experiments on Polistes, our results pave the way for functional genomic research that can contribute significantly to learning the interactions of environment, development, and the roles they play in paper wasp evolution and behavioral ecology.en_US
dc.language.isoengeng
dc.publisherPublic Library of Scienceeng
dc.rightsAttribution CC BYeng
dc.rights.urihttp://creativecommons.org/licenses/by/2.5/eng
dc.titleDevelopment of an RNA Interference Tool, Characterization of Its Target, and an Ecological Test of Caste Differentiation in the Eusocial Wasp Polistesen_US
dc.typePeer reviewed
dc.typeJournal article
dc.description.versionpublishedVersionen_US
dc.rights.holderCopyright 2011 Hunt et al.
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0026641
dc.subject.nsiVDP::Mathematics and natural science: 400::Basic biosciences: 470eng
dc.subject.nsiVDP::Mathematics and natural science: 400::Zoology and botany: 480::Ecology: 488eng


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