dc.contributor.author | Tislevoll, Benedicte Sjo | |
dc.contributor.author | Hellesøy, Monica | |
dc.contributor.author | Fagerholt, Oda Helen Eck | |
dc.contributor.author | Gullaksen, Stein-Erik | |
dc.contributor.author | Srivastava, Aashish | |
dc.contributor.author | Birkeland, Even | |
dc.contributor.author | Kleftogiannis, Dimitrios | |
dc.contributor.author | Ayuda Duran, Pilar | |
dc.contributor.author | Piechaczyk, Laure Isabelle | |
dc.contributor.author | Tadele, Dagim Shiferaw | |
dc.contributor.author | Skavland, Jørn | |
dc.contributor.author | Panagiotis, Baliakas | |
dc.contributor.author | Hovland, Randi | |
dc.contributor.author | Andresen, Vibeke | |
dc.contributor.author | Seternes, Ole Morten | |
dc.contributor.author | Tvedt, Tor Henrik Anderson | |
dc.contributor.author | Aghaeepour, Nima | |
dc.contributor.author | Gavasso, Sonia | |
dc.contributor.author | Porkka, Kimmo | |
dc.contributor.author | Jonassen, Inge | |
dc.contributor.author | Fløisand, Yngvar | |
dc.contributor.author | Enserink, Jorrit | |
dc.contributor.author | Blaser, Nello | |
dc.contributor.author | Gjertsen, Bjørn Tore | |
dc.date.accessioned | 2023-07-10T08:30:54Z | |
dc.date.available | 2023-07-10T08:30:54Z | |
dc.date.created | 2023-03-30T13:11:10Z | |
dc.date.issued | 2023 | |
dc.identifier.issn | 2041-1723 | |
dc.identifier.uri | https://hdl.handle.net/11250/3077440 | |
dc.description.abstract | Aberrant pro-survival signaling is a hallmark of cancer cells, but the response to chemotherapy is poorly understood. In this study, we investigate the initial signaling response to standard induction chemotherapy in a cohort of 32 acute myeloid leukemia (AML) patients, using 36-dimensional mass cytometry. Through supervised and unsupervised machine learning approaches, we find that reduction of extracellular-signal-regulated kinase (ERK) 1/2 and p38 mitogen-activated protein kinase (MAPK) phosphorylation in the myeloid cell compartment 24 h post-chemotherapy is a significant predictor of patient 5-year overall survival in this cohort. Validation by RNA sequencing shows induction of MAPK target gene expression in patients with high phospho-ERK1/2 24 h post-chemotherapy, while proteomics confirm an increase of the p38 prime target MAPK activated protein kinase 2 (MAPKAPK2). In this study, we demonstrate that mass cytometry can be a valuable tool for early response evaluation in AML and elucidate the potential of functional signaling analyses in precision oncology diagnostics. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Nature Research | en_US |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.title | Early response evaluation by single cell signaling profiling in acute myeloid leukemia | en_US |
dc.type | Journal article | en_US |
dc.type | Peer reviewed | en_US |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | Copyright The Author(s) 2023 | en_US |
dc.source.articlenumber | 115 | en_US |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 2 | |
dc.identifier.doi | 10.1038/s41467-022-35624-4 | |
dc.identifier.cristin | 2138498 | |
dc.source.journal | Nature Communications | en_US |
dc.identifier.citation | Nature Communications. 2023, 14, 115. | en_US |
dc.source.volume | 14 | en_US |