XHM: A system for detection of potential cross hybridizations in DNA microarrays
Peer reviewed, Journal article
Peer reviewed
Permanent lenke
https://hdl.handle.net/1956/7174Utgivelsesdato
2004-08-27Metadata
Vis full innførselSamlinger
Originalversjon
https://doi.org/10.1186/1471-2105-5-117Sammendrag
Background: Microarrays have emerged as the preferred platform for high throughput gene expression analysis. Cross-hybridization among genes with high sequence similarities can be a source of error reducing the reliability of DNA microarray results. Results: We have developed a tool called XHM (cross hybridization on microarrays) for assessment of the reliability of hybridization signals by detecting potential cross-hybridizations on DNA microarrays. This is done by comparing the sequences of the probes against an extensive database representing the transcriptome of the organism in question. XHM is available online at http://www.bioinfo.no/tools/xhm/. Conclusions: Using XHM with its user-adjustable parameters will enable scientists to check their lists of differentially expressed genes from microarray experiments for potential crosshybridizations. This provides information that may be useful in the validation of the microarray results.
Utgiver
BioMed CentralTidsskrift
BMC BioinformaticsOpphavsrett
Kristian Flikka et al.; licensee BioMed Central Ltd.Copyright 2004 Flikka et al; licensee BioMed Central Ltd.