dc.contributor.author | Sun, Li-Zhi | en_US |
dc.contributor.author | Elsayed, S. | en_US |
dc.contributor.author | Bronstad, A. M. | en_US |
dc.contributor.author | Van Do, T. | en_US |
dc.contributor.author | Irgens, Ågot | en_US |
dc.contributor.author | Aardal, N. P. | en_US |
dc.contributor.author | Aasen, T. B. | en_US |
dc.date.accessioned | 2012-06-28T07:37:25Z | |
dc.date.available | 2012-06-28T07:37:25Z | |
dc.date.issued | 2007 | eng |
dc.Published | Scandinavian Journal of Immunology 65(2): 118–125. | en |
dc.identifier.issn | 0300-9475 | |
dc.identifier.uri | https://hdl.handle.net/1956/5864 | |
dc.description.abstract | Toluene diisocyanate (TDI), a highly reactive industrial chemical, is one of the leading causes of occupation-related asthma in industrialized countries. The pathogenesis of TDI-induced asthma, however, remains not fully understood, in part due to lack of appropriate animal models. Twenty five female BALB/c mice (age: 8 weeks) were randomly divided into 5 groups: Ovabumin (OVA); OVA peptide amino acid residues No. 323–339 (Pep); TDI; alum and physiological saline. Mice were intraperitoneally injected with 25 lg OVA or pep absorbed on 300 lg alum, 300 lg alum or saline on days 0, 7 and 14. For the TDI group, mice were sensitized subcutaneously with 20 ll neat TDI on day 0; 20 ll of TDI in olive oil (1:10) on days 7 and 14; on days 21–23. Then each group was challenged intranasally with 20 ll of 1% OVA, 1% Pep, 1% TDI, 10% alum and saline respectively. On day 28, mice were killed under pentothal anesthesia. The results demonstrated that neutrophil-dominant inflammation with a few eosinophil infiltration occurred in the peri-bronchial and peri-vascular regions of the lungs. This was accompanied by hyperplasia/ hypertrophy of cells lining the airways and mucus production as shown by HE staining. Positive immunohistochemical MBP staining in parenchyma was also shown. Th2 cytokine IL-4 and IgE production were significant increased 5 days after last challenge while IFN-c level was below the detection limit. Conclusion: the clear elevation of IL-4 and IgE could allow to conclude a possible Th2-like dominated allergic response in TDI-exposed BALB/c mouse model. | en_US |
dc.language.iso | eng | eng |
dc.publisher | Blackwell Publishing Ltd. | eng |
dc.relation.ispartof | <a href="http://hdl.handle.net/1956/5866" target="blank">Toluene diisocyanate (TDI)-induced asthma: Inflammatory and immunological responses to TDI, ovalbumin (OVA) and ovalbumin peptide OVA 323-339 in mouse models</a> | eng |
dc.title | Airway Inflammation and Bronchial Remodelling in Toluene Diisocyanate-exposed BALB/c Mouse Model | en_US |
dc.type | Peer reviewed | |
dc.type | Journal article | |
dc.description.version | acceptedVersion | en_US |
dc.rights.holder | Copyright 2006 The Authors. Journal compilation Copyright 2006 Blackwell Publishing Ltd. Scandinavian Journal of Immunology. | |
dc.identifier.doi | https://doi.org/10.1111/j.1365-3083.2 | |
dc.identifier.cristin | 393891 | |
dc.subject.nsi | VDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710::Medical immunology: 716 | eng |